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Between 1992 and 2022, a long-term increase in\n            <jats:italic>Vibrio<\/jats:italic>\n            spp. infections was reported in Florida, USA. Furthermore, a spike in\n            <jats:italic>Vibrio<\/jats:italic>\n            spp. infections was reported post Hurricane Ian, a category five storm that made landfall in Florida on 28 September 2022. During October 2022, water and oyster samples were collected from three stations in Lee County in an area significantly impacted by Ian.\n            <jats:italic>Vibrio<\/jats:italic>\n            spp. were isolated, and whole-genome sequencing and phylogenetic analysis were done, with a focus on\n            <jats:italic>Vibrio parahaemolyticus<\/jats:italic>\n            and\n            <jats:italic>Vibrio vulnificus<\/jats:italic>\n            to provide genetic insight into pathogenic strains circulating in the environment. Metagenomic analysis of water samples provided insight with respect to human health-related factors, notably the detection of approximately 12 pathogenic\n            <jats:italic>Vibrio<\/jats:italic>\n            spp., virulence and antibiotic resistance genes, and mobile genetic elements, including the SXT\/R391 family of integrative conjugative elements. Environmental parameters were monitored as part of a long-term time series analysis done using satellite remote sensing. In addition to anomalous rainfall and storm surge, changes in sea surface temperature and chlorophyll concentration during and after Ian favored the growth of\n            <jats:italic>Vibrio<\/jats:italic>\n            spp. In conclusion, genetic analysis coupled with environmental data and remote sensing provides useful public health information and, hence, constitute a valuable tool to proactively detect and characterize environmental pathogens, notably vibrios. These data can aid the development of early warning systems by yielding a larger source of information for public health during climate change.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Evidence suggests warming temperatures are associated with the spread of potentially pathogenic\n              <jats:italic>Vibrio<\/jats:italic>\n              spp. and the emergence of human disease globally. Following Hurricane Ian, the State of Florida reported a sharp increase in the number of reported\n              <jats:italic>Vibrio<\/jats:italic>\n              spp. infections and deaths. Hence, monitoring of pathogens, including vibrios, and environmental parameters influencing their occurrence is critical to public health. Here, DNA sequencing was used to investigate the genomic diversity of\n              <jats:italic>Vibrio parahaemolyticus<\/jats:italic>\n              and\n              <jats:italic>Vibrio vulnificus<\/jats:italic>\n              , both potential human pathogens, in Florida coastal waters post Hurricane Ian, in October 2022. Additionally, the microbial community of water samples was profiled to detect the presence of\n              <jats:italic>Vibrio<\/jats:italic>\n              spp. and other microorganisms (bacteria, fungi, protists, and viruses) present in the samples. Long-term environmental data analysis showed changes in environmental parameters during and after Ian were optimal for the growth of\n              <jats:italic>Vibrio<\/jats:italic>\n              spp. and related pathogens. Collectively, results will be used to develop predictive risk models during climate change.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.01476-23","type":"journal-article","created":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T13:01:11Z","timestamp":1697461271000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Genomic diversity of\n            <i>Vibrio<\/i>\n            spp. and metagenomic analysis of pathogens in Florida Gulf coastal waters following Hurricane Ian"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3234-3337","authenticated-orcid":true,"given":"Kyle D.","family":"Brumfield","sequence":"first","affiliation":[{"name":"Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, USA"},{"name":"University of Maryland Institute for Advanced Computer Studies, University of Maryland, College Park, 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