{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:22:17Z","timestamp":1788884537033,"version":"build-2803163510"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["220266\/Z\/20\/Z"],"award-info":[{"award-number":["220266\/Z\/20\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["222323\/Z\/21\/Z"],"award-info":[{"award-number":["222323\/Z\/21\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000654","name":"Marie Curie","doi-asserted-by":"publisher","award":["955910"],"award-info":[{"award-number":["955910"]}],"id":[{"id":"10.13039\/501100000654","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/W004453\/1"],"award-info":[{"award-number":["EP\/W004453\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,8,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Malaria kills over 600,000 people annually, with all clinical symptoms arising from blood-stage infection.\n                      <jats:italic toggle=\"yes\">Plasmodium falciparum<\/jats:italic>\n                      blood-stage replication happens primarily in the blood circulation, bone marrow, and spleen, where there are flow-generated forces, yet most\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      growth assays are carried out in static conditions. We systematically tested the effect of orbital shaking on growth and linked it to the wall shear stress forces generated by the resultant fluid motion. Strikingly, there is a critical shaking speed, below which growth rates are reduced and above which growth increases. Forces at this critical speed correspond to previously measured forces in the microvasculature. Red blood cell invasion depends on two families of parasite attachment proteins, the\n                      <jats:italic toggle=\"yes\">Plasmodium falciparum<\/jats:italic>\n                      erythrocyte-binding antigen and the\n                      <jats:italic toggle=\"yes\">Plasmodium falciparum<\/jats:italic>\n                      reticulocyte-binding protein. Using a panel of knockouts, we show for the first time that several of these ligands have greater importance in high wall shear stress conditions, highlighting the importance of understanding the effect of fluid motion on parasite biology.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Malaria parasite growth occurs in dynamic environments like blood circulation, where fluid forces impact red blood cells and parasites. Yet, most laboratory growth assays are conducted in static environments, failing to replicate these forces. We explored the effects of growing the parasites on orbital shakers, which generate biologically relevant forces, and found that shaking speed critically impacts parasite growth, with reduced growth at speeds that mimic forces in the microvasculature. Importantly, using these conditions revealed invasion phenotypes not observed under static conditions. Understanding how fluid dynamics influence parasite growth offers a new approach to investigating malaria pathogenesis, with the potential to improve the development of therapeutic interventions.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.01499-25","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T09:00:39Z","timestamp":1750669239000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Under conditions of high wall shear stress, several PfEBA and PfRH ligands are important for malaria\n                    <i>Plasmodium falciparum<\/i>\n                    blood-stage growth"],"prefix":"10.1128","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2321-3661","authenticated-orcid":false,"given":"Emma","family":"Kals","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"Cambridge Institute for Medical Research, University of Cambridge","place":["Cambridge, United Kingdom"]},{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"Cavendish Laboratory, University of Cambridge","place":["Cambridge, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten","family":"Kals","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"Cavendish Laboratory, University of Cambridge","place":["Cambridge, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Cicuta","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"Cavendish Laboratory, University of Cambridge","place":["Cambridge, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9835-1014","authenticated-orcid":false,"given":"Julian C.","family":"Rayner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"Cambridge Institute for Medical Research, University of Cambridge","place":["Cambridge, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.09.007"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu203"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008587"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.43.30"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3008882.Plasmodium"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2007.01881.x"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.4.1986-1994.2005"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2019.04.210"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(82)90202-4"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.2307\/3281094"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2009.09.005"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069781"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-24218-0"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00534.x"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7015505"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-004-1266-6"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.006"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004670"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10606"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.06.004"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1115257"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2011.10.002"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32138"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003943"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","unstructured":"Ahouidi AD Amambua-ngwa A Awandare GA Bei AK Conway DJ Diakite M Duraisingh MT Rayner JC. 2016. 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