{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T13:54:18Z","timestamp":1791554058661,"version":"4.3.4"},"reference-count":70,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Canadian Government | Canadian Institutes of Health Research","award":["FDN-154288"],"award-info":[{"award-number":["FDN-154288"]}],"id":[{"id":"https:\/\/ror.org\/01gavpb45","id-type":"ROR","asserted-by":"crossref"}]},{"name":"HHS | National Institutes of Health","award":["R01AI127375"],"award-info":[{"award-number":["R01AI127375"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2024,8,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      At human body temperature, the fungal pathogen\n                      <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                      can transition from yeast to filamentous morphologies in response to host-relevant cues. Additionally, elevated temperatures encountered during febrile episodes can independently induce\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      filamentation. However, the underlying genetic pathways governing this developmental transition in response to elevated temperatures remain largely unexplored. Here, we conducted a functional genomic screen to unravel the genetic mechanisms orchestrating\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      filamentation specifically in response to elevated temperature, implicating 45% of genes associated with the spliceosome or pre-mRNA splicing in this process. Employing RNA-Seq to elucidate the relationship between mRNA splicing and filamentation, we identified greater levels of intron retention in filaments compared to yeast, which correlated with reduced expression of the affected genes. Intriguingly, homozygous deletion of a gene encoding a spliceosome component important for filamentation (\n                      <jats:italic toggle=\"yes\">PRP19<\/jats:italic>\n                      ) caused even greater levels of intron retention compared with wild type and displayed globally dysregulated gene expression. This suggests that intron retention is a mechanism for fine-tuning gene expression during filamentation, with perturbations of the spliceosome exacerbating this process and blocking filamentation. Overall, this study unveils a novel biological process governing\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      filamentation, providing new insights into the complex regulation of this key virulence trait.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Fungal pathogens such as\n                        <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                        can cause serious infections with high mortality rates in immunocompromised individuals. When\n                        <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                        is grown at temperatures encountered during human febrile episodes, yeast cells undergo a transition to filamentous cells, and this process is key to its virulence. Here, we expanded our understanding of how\n                        <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                        undergoes filamentation in response to elevated temperature and identified many genes involved in mRNA splicing that positively regulate filamentation. Through transcriptome analyses, we found that intron retention is a mechanism for fine-tuning gene expression in filaments, and perturbation of the spliceosome exacerbates intron retention and alters gene expression substantially, causing a block in filamentation. This work adds to the growing body of knowledge on the role of introns in fungi and provides new insights into the cellular processes that regulate a key virulence trait in\n                        <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.01535-24","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T09:00:39Z","timestamp":1720515639000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["The spliceosome impacts morphogenesis in the human fungal pathogen\n                    <i>Candida albicans<\/i>"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0676-3215","authenticated-orcid":false,"given":"Emma","family":"Lash","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics, University of Toronto","place":["Toronto, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Maufrais","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Biologie des ARN des Pathog\u00e8nes Fongiques, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9","place":["Paris, France"]},{"name":"HUB Bioinformatique et Biostatistique, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilhem","family":"Janbon","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Biologie des ARN des Pathog\u00e8nes Fongiques, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2821-9269","authenticated-orcid":false,"given":"Nicole","family":"Robbins","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Toronto","place":["Toronto, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8965-290X","authenticated-orcid":false,"given":"Lydia","family":"Herzel","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Biochemistry, Freie Universit\u00e4t Berlin","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5797-0110","authenticated-orcid":false,"given":"Leah E.","family":"Cowen","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Toronto","place":["Toronto, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(23)00692-8"},{"key":"e_1_3_5_3_2","unstructured":"World Health Organization. 2022. 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