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CA-CA domain interactions are responsible for multimerization of the Gag polyprotein at virus assembly sites, and in the mature virion, CA monomers assemble into a conical core that encapsidates the viral RNA genome. Multiple CA regions that contribute to the assembly and release of HIV-1 particles have been mapped and investigated. Here, we identified and characterized a Pro-rich loop in CA that is important for the formation of the immature Gag lattice. Changes in this region disrupt viral production and abrogate the formation of infectious, mature virions. Propagation of the mutants in culture led to the selection of second-site compensatory mutations within CA. These results expand our knowledge of the assembly and maturation steps in the viral replication cycle and may be relevant for development of antiviral drugs targeting CA.<\/jats:p>","DOI":"10.1128\/mbio.01567-18","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T09:25:11Z","timestamp":1539595511000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Identification of a Structural Element in HIV-1 Gag Required for Virus Particle Assembly and Maturation"],"prefix":"10.1128","volume":"9","author":[{"given":"Mariia","family":"Novikova","sequence":"first","affiliation":[{"name":"Virus-Cell Interaction Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas J.","family":"Adams","sequence":"additional","affiliation":[{"name":"Laboratory 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