{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T19:39:13Z","timestamp":1793129953986,"version":"4.5.0"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI145939"],"award-info":[{"award-number":["R01AI145939"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM119197"],"award-info":[{"award-number":["R01GM119197"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM083016"],"award-info":[{"award-number":["R01GM083016"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI173607"],"award-info":[{"award-number":["R21AI173607"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["F30AI191694"],"award-info":[{"award-number":["F30AI191694"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM140935"],"award-info":[{"award-number":["T32GM140935"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2026,10,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Systemic\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">Candidozyma<\/jats:italic>\n                      )\n                      <jats:italic toggle=\"yes\">auris<\/jats:italic>\n                      infections can have mortality rates up to 60%, and little is known about immune responses to this fungal pathogen. Neutrophils play a major role in immunity to many fungal pathogens, including\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      species. However, neutrophils internalize\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      at a lower rate than\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      . We previously found that the\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      cell wall mannan blocks access to \u03b2-glucan, an immunostimulatory motif. Here, we analyzed the immune-evasion properties of\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      mannan separate from glucan masking. We generated eight mannosylation mutants and identified two with increased rates of phagocytosis by human neutrophils. One of these mutants,\n                      <jats:italic toggle=\"yes\">mnn1<\/jats:italic>\n                      \u0394, did not exhibit increased exposure of \u03b2-glucan. Nuclear magnetic resonance analysis of\n                      <jats:italic toggle=\"yes\">mnn1<\/jats:italic>\n                      \u0394 mannan showed decreased \u03b1-1,3-mannose linkages and acid-labile side chains. These structural changes increased the affinity of human Dectin-2 and Mincle for mannans\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      , and Fab blocking of Dectin-2 decreased human neutrophil phagocytosis of\n                      <jats:italic toggle=\"yes\">mnn1<\/jats:italic>\n                      \u0394. Overall, our work provides insight into human neutrophil recognition of\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      mannans and further understanding of mechanisms of immune evasion for this fungal pathogen.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Cell surface glycan recognition plays an important role in the innate immune response to fungal pathogens. Here, we use\n                        <jats:italic toggle=\"yes\">Candida auris<\/jats:italic>\n                        mannosylation mutants to identify\n                        <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                        genes that contribute to its neutrophil evasion properties. We identify a mannosylation gene,\n                        <jats:italic toggle=\"yes\">MNN1<\/jats:italic>\n                        , that contributes to human and murine peripheral blood neutrophil evasion without significantly impacting beta-glucan masking. We attribute\n                        <jats:italic toggle=\"yes\">MNN1<\/jats:italic>\n                        to the alteration of cell surface mannans that leads to decreased Dectin-2-binding affinity. While mannan has been associated with masking \u03b2-glucan in the\n                        <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                        cell wall, the impact of specific mannan structures on receptor binding presents an additional mechanism of neutrophil evasion for\n                        <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.01701-26","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:02:18Z","timestamp":1790254938000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["<i>Candida auris MNN1<\/i>\n                    contributes to human neutrophil evasion via mechanisms independent of \u03b2-glucan 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