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Cryptosporidiosis is of particular importance in infants and shows a strong association with malnutrition, both as a risk factor and as a consequence.\n                      <jats:italic toggle=\"yes\">Cryptosporidium<\/jats:italic>\n                      invades and replicates within the small intestine epithelial cells. This is a highly dynamic tissue that is developmentally stratified along the villus axis. New cells emerge from a stem cell niche in the crypt and differentiate into mature epithelial cells while moving toward the villus tip, where they are ultimately shed. Here, we studied the impact of\n                      <jats:italic toggle=\"yes\">Cryptosporidium<\/jats:italic>\n                      infection on this dynamic architecture. Tracing DNA synthesis in pulse-chase experiments\n                      <jats:italic toggle=\"yes\">in vivo,<\/jats:italic>\n                      we quantified the genesis and migration of epithelial cells along the villus. We found proliferation and epithelial migration to be elevated in response to\n                      <jats:italic toggle=\"yes\">Cryptosporidium<\/jats:italic>\n                      infection. Infection also resulted in significant cell loss documented by imaging and molecular assays. Consistent with these observations, single-cell RNA sequencing of infected intestines showed a gain of young and a loss of mature cells. Interestingly, enhanced epithelial cell loss was not a function of enhanced apoptosis of infected cells. To the contrary,\n                      <jats:italic toggle=\"yes\">Cryptosporidium<\/jats:italic>\n                      -infected cells were less likely to be apoptotic than bystanders, and experiments in tissue culture demonstrated that infection provided enhanced resistance to chemically induced apoptosis to the host but not bystander cells. Overall, this study suggests that\n                      <jats:italic toggle=\"yes\">Cryptosporidium<\/jats:italic>\n                      may modulate cell apoptosis and documents pronounced changes in tissue homeostasis due to parasite infection, which may contribute to its long-term impact on the developmental and nutritional state of children.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      The intestine must balance its roles in digestion and nutrient absorption with the maintenance of an effective barrier to colonization and breach by numerous potential pathogens. An important component of this balance is its constant turnover, which is modulated by a gain of cells due to proliferation and loss due to death or extrusion. Here, we report that\n                      <jats:italic toggle=\"yes\">Cryptosporidium<\/jats:italic>\n                      infection changes the dynamics of this process increasing both gain and loss of enterocytes speeding up the villus elevator. This leads to a much more immature epithelium and a reduction of the number of those cells typically found toward the villus apex best equipped to take up key nutrients including carbohydrates and lipids. These changes in the cellular architecture and physiology of the small intestine may be linked to the profound association between cryptosporidiosis and malnutrition.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.01720-24","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T09:01:17Z","timestamp":1720774877000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["<i>Cryptosporidium<\/i>\n                    impacts epithelial turnover and is resistant to induced death of the host cell"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6432-2135","authenticated-orcid":false,"given":"Bethan A.","family":"Wallbank","sequence":"first","affiliation":[{"name":"Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania","place":["Philadelphia, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan D.","family":"Pardy","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania","place":["Philadelphia, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7970-872X","authenticated-orcid":true,"given":"Igor E.","family":"Brodsky","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania","place":["Philadelphia, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3092-1428","authenticated-orcid":true,"given":"Christopher A.","family":"Hunter","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania","place":["Philadelphia, 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