{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T01:29:21Z","timestamp":1778808561066,"version":"3.51.4"},"reference-count":95,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2021,1,19]],"date-time":"2021-01-19T00:00:00Z","timestamp":1611014400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000185","name":"DOD | Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","award":["D17AC00009"],"award-info":[{"award-number":["D17AC00009"]}],"id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015326","name":"HHS | NIH | OSC | Common Fund","doi-asserted-by":"publisher","award":["DP1 OD017181"],"award-info":[{"award-number":["DP1 OD017181"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2021,2,23]]},"abstract":"<jats:p>\n                    Recent studies have renewed interest in developing novel antiviral therapeutics and vaccines based on defective interfering particles (DIPs)\u2014a subset of viral deletion mutants that conditionally replicate. Identifying and engineering DIPs require that viral\n                    <jats:italic>cis<\/jats:italic>\n                    - and\n                    <jats:italic>trans<\/jats:italic>\n                    -acting elements be accurately mapped.\n                  <\/jats:p>","DOI":"10.1128\/mbio.01724-20","type":"journal-article","created":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T08:01:03Z","timestamp":1610956863000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["RanDeL-Seq: a High-Throughput Method to Map Viral\n                    <i>cis<\/i>\n                    - and\n                    <i>trans<\/i>\n                    -Acting Elements"],"prefix":"10.1128","volume":"12","author":[{"given":"Timothy","family":"Notton","sequence":"first","affiliation":[{"name":"Gladstone Center for Cell Circuitry, Gladstone Institutes, San Francisco, California, USA"},{"name":"University of California, Berkeley, San Francisco Joint Graduate Group in Bioengineering, San Francisco, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua J.","family":"Glazier","sequence":"additional","affiliation":[{"name":"Gladstone Center for Cell Circuitry, Gladstone Institutes, San Francisco, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria R.","family":"Saykally","sequence":"additional","affiliation":[{"name":"Gladstone Center for Cell Circuitry, Gladstone Institutes, San Francisco, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cassandra E.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Gladstone Center for Cell Circuitry, Gladstone Institutes, San Francisco, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9987-6357","authenticated-orcid":false,"given":"Leor S.","family":"Weinberger","sequence":"additional","affiliation":[{"name":"Gladstone Center for Cell Circuitry, Gladstone Institutes, San Francisco, California, USA"},{"name":"Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-2836(61)80072-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3288"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.02.032"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1756"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2009.09.007"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02582"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00645"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1373"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02569-09"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02075-15"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02490-12"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2012.1.1.35"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-018-0175-7"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1542"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005986"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/226325a0"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0065-3527(08)60529-1"},{"key":"e_1_3_2_19_2","volume-title":"Fields virology","author":"Knipe DM","year":"2013","unstructured":"Knipe DM, Howley PM. 2013. Fields virology, 6th ed. Lippincott Williams & Wilkins, Philadelphia, PA.","edition":"6"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.98.2534.87"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-5933-y"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02921-15"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2018.07.014"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01786-18"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03193-13"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00743-08"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2019.04.006"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8657-6_2"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx030"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E16-08-0612"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4264"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006824"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3490"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky152"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku981"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.4.3386-3403.1999"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.74.10.4590-4600.2000"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01243-z"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00484-17"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.735613"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.01.007"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.3.2265-2270.2003"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.68.12.8131-8140.1994"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1006\/mthe.2002.0672"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl349"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.64.1.185-194.1990"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku509"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.04.054"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1320524110"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02406-16"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.02.007"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14279"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4048"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.13.7367"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-755-6:083"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2005.01.027"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1086\/280418"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.es.09.110178.000335"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.04120"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.04.005"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.8.4685-4694.2003"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01952-09"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80828-4"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.65.5.2415-2421.1991"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00974-06"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00723-10"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0010018"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.76.23.12087-12096.2002"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00203-07"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks753"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2018.01.011"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.67.11.6365-6378.1993"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00246-16"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018197"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.10.034"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03745-13"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.2174\/157016206775197655"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048688"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.20.5748"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00243-13"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00654-08"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.77.18.10004-10014.2003"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.70.6.3930-3937.1996"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.234302"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000182"},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00698-17"},{"key":"e_1_3_2_87_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302623"},{"key":"e_1_3_2_88_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature20124"},{"key":"e_1_3_2_89_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00354-19"},{"key":"e_1_3_2_90_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306764101"},{"key":"e_1_3_2_91_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.59.2.284-291.1986"},{"key":"e_1_3_2_92_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(85)90120-9"},{"key":"e_1_3_2_93_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050859"},{"key":"e_1_3_2_94_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_2_95_2","doi-asserted-by":"publisher","DOI":"10.3791\/52065"},{"key":"e_1_3_2_96_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14121"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.01724-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.01724-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T22:23:00Z","timestamp":1646691780000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.01724-20"}},"subtitle":[],"editor":[{"given":"Stacy M.","family":"Horner","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,2,23]]},"references-count":95,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,2,23]]}},"alternative-id":["10.1128\/mBio.01724-20"],"URL":"https:\/\/doi.org\/10.1128\/mbio.01724-20","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.07.01.183574","asserted-by":"object"}]},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,23]]},"assertion":[{"value":"2020-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01724-20"}}