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Bacteriolytic enzymes are promising new antimicrobials because they rapidly kill bacteria, including drug-resistant and persisting cells, by destroying their cell wall. However, when injected into the bloodstream, these enzymes are not retained long enough to clear an infection. Here, we describe a modification to increase blood circulation time of the enzymes and enhance treatment efficacy against\n                    <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n                    -induced bloodstream infections. This was achieved by preselecting enzyme candidates for high activity in human blood and coupling them to serum albumin, thereby preventing their elimination by kidney filtration and blood vessel cells.\n                  <\/jats:p>","DOI":"10.1128\/mbio.01781-20","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T13:30:23Z","timestamp":1600695023000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Engineering of Long-Circulating Peptidoglycan Hydrolases Enables Efficient Treatment of Systemic Staphylococcus aureus Infection"],"prefix":"10.1128","volume":"11","author":[{"given":"Anna M.","family":"Sobieraj","sequence":"first","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4308-1619","authenticated-orcid":false,"given":"Markus","family":"Huemer","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9a V.","family":"Zinsli","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Meile","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja P.","family":"Keller","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"R\u00f6hrig","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fritz","family":"Eichenseher","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Shen","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4700-1118","authenticated-orcid":false,"given":"Annelies S.","family":"Zinkernagel","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8162-2631","authenticated-orcid":false,"given":"Martin J.","family":"Loessner","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3535-3861","authenticated-orcid":false,"given":"Mathias","family":"Schmelcher","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00134-14"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(05)70295-4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05022-11"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2015.1100533"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1212788"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2008.11.005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2200"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/472032a"},{"key":"e_1_3_2_10_2","volume-title":"Antibiotic resistance threats in the United States, 2019.","author":"CDC","year":"2019","unstructured":"CDC. 2019. 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