{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:35:37Z","timestamp":1790235337907,"version":"4.1.0"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T00:00:00Z","timestamp":1566864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/AuthorWarrantyLicense.v1"},{"start":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T00:00:00Z","timestamp":1566864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2019,8,27]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">Cryptococcus gattii<\/jats:named-content>\n            , one of the etiological agents of cryptococcosis, can be distinguished from its sister species\n            <jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>\n            by growth on\n            <jats:sc>d<\/jats:sc>\n            -amino acids.\n            <jats:named-content content-type=\"genus-species\">C. gattii<\/jats:named-content>\n            <jats:italic>MYO5<\/jats:italic>\n            affected the growth of\n            <jats:named-content content-type=\"genus-species\">C. gattii<\/jats:named-content>\n            on\n            <jats:sc>d<\/jats:sc>\n            -amino acids. The\n            <jats:italic>myo5\u0394<\/jats:italic>\n            cells accumulated high levels of various substrates from outside the cells, and excessively accumulated\n            <jats:sc>d<\/jats:sc>\n            -amino acids appeared to have caused toxicity in the\n            <jats:italic>myo5\u0394<\/jats:italic>\n            cells. We provide evidence on the alteration of membrane properties in the\n            <jats:italic>myo5\u0394<\/jats:italic>\n            mutants. Additionally, alteration in the\n            <jats:italic>myo5\u0394<\/jats:italic>\n            membrane permeability causing higher substrate accumulation is associated with the changes in the sterol distribution. Furthermore, myosin-I in three other yeasts also manifested a similar role in substrate accumulation. Thus, while fungal myosin-I may function as a classical myosin-I, it has hitherto unknown additional roles in regulating membrane permeability. Since deletion of fungal myosin-I causes significantly elevated susceptibility to multiple antifungal drugs, it could serve as an effective target for augmentation of fungal therapy.\n          <\/jats:p>","DOI":"10.1128\/mbio.01867-19","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T09:40:48Z","timestamp":1566812448000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["A Novel Role of Fungal Type I Myosin in Regulating Membrane Properties and Its Association with\n            <scp>d<\/scp>\n            -Amino Acid Utilization in Cryptococcus gattii"],"prefix":"10.1128","volume":"10","author":[{"given":"Ami","family":"Khanal Lamichhane","sequence":"first","affiliation":[{"name":"Molecular Microbiology Section, Laboratory of Clinical Immunology and Microbiology, NIAID, NIH, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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