{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T11:25:36Z","timestamp":1785151536055,"version":"3.55.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["1R01AI146103"],"award-info":[{"award-number":["1R01AI146103"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007699","name":"University of Georgia","doi-asserted-by":"crossref","award":["Startup funds"],"award-info":[{"award-number":["Startup funds"]}],"id":[{"id":"10.13039\/100007699","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2024,9,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Major\n              <jats:italic>Candida albicans<\/jats:italic>\n              virulence traits include its ability to make hyphae, to produce a biofilm, and to damage host cells. These traits depend upon expression of hypha-associated genes. A gene expression comparison among clinical isolates suggested that transcription factor Rme1\n              <jats:italic>,<\/jats:italic>\n              established by previous studies to be a positive regulator of chlamydospore formation, may also be a negative regulator of hypha-associated genes. Engineered\n              <jats:italic>RME1<\/jats:italic>\n              overexpression supported this hypothesis, but no relevant\n              <jats:italic>rme1<\/jats:italic>\n              \u0394\/\u0394 mutant phenotype was detected. We reasoned that Rme1 may function within a specific regulatory pathway. This idea was supported by our finding that an\n              <jats:italic>rme1<\/jats:italic>\n              \u0394\/\u0394 mutation relieves the need for biofilm regulator Brg1 in biofilm formation. The impact of the\n              <jats:italic>rme1<\/jats:italic>\n              \u0394\/\u0394 mutation is most prominent under static or \u201cbiofilm-like\u201d growth conditions. RNA sequencing (RNA-seq) of cells grown under biofilm-like conditions indicates that Brg1 activates hypha-associated genes indirectly via repression of\n              <jats:italic>RME1<\/jats:italic>\n              : hypha-associated gene expression levels are substantially reduced in a\n              <jats:italic>brg1<\/jats:italic>\n              \u0394\/\u0394 mutant and partially restored in a\n              <jats:italic>brg1<\/jats:italic>\n              \u0394\/\u0394\n              <jats:italic>rme1<\/jats:italic>\n              \u0394\/\u0394 double mutant. An\n              <jats:italic>rme1<\/jats:italic>\n              \u0394\/\u0394 mutation does not simply bypass Brg1, because iron homeostasis genes depend upon Brg1 regardless of Rme1. Rme1 thus connects Brg1 to the targets relevant to hypha and biofilm formation under biofilm growth conditions.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>Candida albicans<\/jats:italic>\n                is a major fungal pathogen of humans, and its ability to grow as a surface-associated biofilm on implanted devices is a common cause of infection. Here, we describe a new regulator of biofilm formation,\n                <jats:italic>RME1<\/jats:italic>\n                , whose activity is most prominent under biofilm-like growth conditions.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/mbio.01872-24","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T13:02:25Z","timestamp":1722344545000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["A Brg1-Rme1 circuit in\n            <i>Candida albicans<\/i>\n            hyphal gene regulation"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7383-743X","authenticated-orcid":false,"given":"Min-Ju","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Georgia, Athens, Georgia, 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