{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:15:43Z","timestamp":1792023343100,"version":"4.4.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1800304"],"award-info":[{"award-number":["2022YFD1800304"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"SKLADCP","award":["SKLADCP2025HP03"],"award-info":[{"award-number":["SKLADCP2025HP03"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172846"],"award-info":[{"award-number":["32172846"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32430106"],"award-info":[{"award-number":["32430106"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Joint Research Foundation of Gansu Province","award":["23JRRA1476"],"award-info":[{"award-number":["23JRRA1476"]}]},{"name":"Joint Research Foundation of Gansu Province","award":["23JRRA1480"],"award-info":[{"award-number":["23JRRA1480"]}]},{"name":"Major Scientific and Technological Special Project of Gansu Province","award":["22ZD6NA001"],"award-info":[{"award-number":["22ZD6NA001"]}]},{"name":"Major Scientific and Technological Special Project of Gansu Province","award":["23ZDNA007"],"award-info":[{"award-number":["23ZDNA007"]}]},{"name":"Major Scientific and Technological Special Project of Gansu Province","award":["24ZD13NA008"],"award-info":[{"award-number":["24ZD13NA008"]}]},{"name":"Basic Research Center, Innovation Program of Chinese Academy of Agricultural Sciences","award":["CAAS-BRC-LPDC-2025-02"],"award-info":[{"award-number":["CAAS-BRC-LPDC-2025-02"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,10,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Secondary infection is a worldwide problem in the prevention and control of viral infection. Secondary bacterial infection induced by porcine reproductive and respiratory syndrome virus (PRRSV) infection causes enormous economic losses, but the relevant mechanism remains unclear. We found that the infection of\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      or\n                      <jats:italic toggle=\"yes\">Streptococcus suis type 2<\/jats:italic>\n                      in the lungs of PRRSV-challenged piglets was significantly higher than the controls, and the infection of PRRSV, influenza A virus H1N1 (H1N1), and porcine circovirus type 2 (PCV2) also significantly increased the infection of the bacteria\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      . Transcriptomic analysis revealed that PRRSV infection significantly altered the expression of cytoskeleton-related proteins, among which the expression of actin-binding protein filamin A (FLNA) was significantly increased, and knockdown of FLNA could significantly reduce bacterial invasion. Mechanistic studies found that FLNA drives actin cytoskeleton rearrangement by promoting F-actin generation, thereby facilitating bacterial invasion. Further studies found that PRRSV promoted bacterial adhesion by upregulating the expression of integrin \u03b15 (ITG\u03b15). ITG\u03b15 could induce actin cytoskeleton rearrangement by promoting FLNA expression, thus aggravating bacterial invasion. Furthermore, we found that lentiviral shRNA-mediated knockdown of FLNA or ITG\u03b15 significantly reduced bacterial infection in the lungs of mice and protected mice from death. These results suggest that the regulation of actin cytoskeleton and cell membrane proteins may be a conserved mechanism of virus-induced secondary bacterial infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>An important reason why porcine reproductive and respiratory syndrome virus (PRRSV) is difficult to control effectively is that it often causes severe secondary bacterial infections, which are usually attributed to the immunosuppression caused by PRRSV. However, the mechanism by which PRRSV infection leads to increased susceptibility of cells to bacterial infection has been largely overlooked. We revealed that PRRSV induced actin cytoskeleton rearrangement by upregulating FLNA expression, thereby aggravating bacterial invasion. PRRSV increased bacterial adhesion by promoting the ITG\u03b15 expression, and the upregulation of ITG\u03b15 could induce FLNA-mediated actin cytoskeleton rearrangement. Furthermore, we found that H1N1 and porcine circovirus type 2 infection also significantly promoted the expression of FLNA and ITG\u03b15 and increased the infection of multiple bacteria. These results suggest that FLNA and ITG\u03b15 play important roles in virus-induced secondary bacterial infection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.01945-25","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T11:51:21Z","timestamp":1757677881000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["PRRSV promotes bacterial infection by remodeling actin cytoskeleton and cell membrane proteins"],"prefix":"10.1128","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0038-5046","authenticated-orcid":false,"given":"Xiao","family":"Liu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01mkqqe32","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of 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