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In this article, we describe a neuronal cell culture model (SH-SY5Y cells) in which a subset of contemporary 2014 outbreak strains of EV-D68 show infectivity in neuronal cells, or neurotropism. We confirmed the difference in neurotropism\n                    <jats:italic>in vitro<\/jats:italic>\n                    using primary human neuron cell cultures and\n                    <jats:italic>in vivo<\/jats:italic>\n                    with a mouse paralysis model. Using the SH-SY5Y cell model, we determined that a barrier to viral entry is at least partly responsible for neurotropism. SH-SY5Y cells may be useful in determining if specific EV-D68 genetic determinants are associated with neuropathogenesis, and replication in this cell line could be used as rapid screening tool for identification of neurotropic EV-D68 strains. This may assist with better understanding of pathogenesis and epidemiology and with the development of potential therapies.\n                  <\/jats:p>","DOI":"10.1128\/mbio.01954-18","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T05:25:11Z","timestamp":1539581111000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":105,"title":["Contemporary Circulating Enterovirus D68 Strains Have Acquired the Capacity for Viral Entry and Replication in Human Neuronal Cells"],"prefix":"10.1128","volume":"9","author":[{"given":"David M.","family":"Brown","sequence":"first","affiliation":[{"name":"J. 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