{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T10:04:54Z","timestamp":1786701894835,"version":"build-2736575974"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP 1217093"],"award-info":[{"award-number":["OPP 1217093"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014496","name":"Stanford Center for Innovation in Global Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014496","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIH R01","award":["R01 AI181283"],"award-info":[{"award-number":["R01 AI181283"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,9,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Typhoid fever remains a global public health challenge, especially in low- and middle-income countries where poor sanitation and limited access to clean water facilitate transmission. The lack of data on disease burden poses a significant barrier to adopting effective interventions such as vaccination programs. We developed a novel colorimetric assay for the detection of\n                      <jats:italic toggle=\"yes\">S<\/jats:italic>\n                      . Typhi-specific bacteriophages (phages) in environmental water samples, providing an indirect indicator of\n                      <jats:italic toggle=\"yes\">S<\/jats:italic>\n                      . Typhi contamination and insights into typhoid burden. We collected surface water samples from Brazil, C\u00f4te d\u2019Ivoire, Nepal, and Niger, covering urban, peri-urban, and rural areas. We evaluated the colorimetric assay efficiency against the agar overlay plaque assay. Isolated phages were tested against various bacteria to assess their host range. The colorimetric assay demonstrated high sensitivity (100% concordance with double agar overlay) with a detection limit of 28 plaque-forming units per milliliter (PFU\/mL), and results were obtained in 5.5 hours. Phage detection rates were highest in densely populated areas with poor sanitation, particularly in Kathmandu, Nepal (98% positivity in river samples) and Abidjan, C\u00f4te d\u2019Ivoire (35.1% positivity in drainage samples). The detection of\n                      <jats:italic toggle=\"yes\">S<\/jats:italic>\n                      . Typhi phages in C\u00f4te d\u2019Ivoire is particularly important, as the burden of typhoid in the region was previously undocumented. Phages were not detected in rural and drinking water sources. Host range analyses demonstrated that all isolated phages were specific to\n                      <jats:italic toggle=\"yes\">S<\/jats:italic>\n                      . Typhi. The novel colorimetric assay offers a rapid and sensitive method for detecting\n                      <jats:italic toggle=\"yes\">S<\/jats:italic>\n                      . Typhi bacteriophages in environmental water. The scalability, low cost (~$2.40 per sample), and minimal equipment requirements suggest that this could be an effective tool for typhoid surveillance in resource-limited settings.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Typhoid fever, caused by\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      Typhi, remains a significant global health threat, particularly in low-resource settings with inadequate sanitation. Effective control measures, such as vaccines, require precise data on where typhoid is most prevalent, yet current surveillance methods are expensive and limited in scope. This study introduces a rapid, low-cost, and scalable colorimetric assay for detecting S. Typhi bacteriophages (viruses that infect bacteria) in environmental water samples. Unlike traditional methods, this test detects the presence of S. Typhi indirectly by identifying associated phages, offering a specific and sensitive approach for monitoring typhoid fever circulation. The assay was validated in diverse settings across four countries and demonstrated high accuracy and cost efficiency. By reducing reliance on expensive laboratory equipment and complex procedures, this tool makes typhoid surveillance more accessible, especially in low-income regions, helping prioritize vaccination campaigns and improve public health interventions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.01963-25","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T12:00:27Z","timestamp":1755518427000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Rapid, low-cost colorimetric detection of\n                    <i>Salmonella<\/i>\n                    Typhi bacteriophages for environmental surveillance"],"prefix":"10.1128","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8593-1119","authenticated-orcid":true,"given":"Kesia Esther","family":"da 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