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The tsetse fly vector is of interest for its potential to prevent disease spread, as it is essential for\n            <jats:named-content content-type=\"genus-species\">T. brucei<\/jats:named-content>\n            life cycle progression and transmission. The tsetse\u2019s mutualistic endosymbiont\n            <jats:named-content content-type=\"genus-species\">Sodalis glossinidius<\/jats:named-content>\n            has a link to trypanosome establishment, providing a disease control target. Here, we describe a new, experimentally verified model of\n            <jats:named-content content-type=\"genus-species\">S. glossinidius<\/jats:named-content>\n            metabolism. This model has enabled the development of a defined growth medium that was used successfully to test aspects of\n            <jats:italic>S. glossinidius<\/jats:italic>\n            metabolism. We present\n            <jats:italic>S. glossinidius<\/jats:italic>\n            as uniquely adapted to life in the tsetse, through its reliance on the blood diet and host-derived sugars. Additionally,\n            <jats:italic>S. glossinidius<\/jats:italic>\n            has adapted to the tsetse\u2019s obligate symbiont\n            <jats:named-content content-type=\"genus-species\">Wigglesworthia glossinidia<\/jats:named-content>\n            by scavenging a vitamin it produces for the insect. This work highlights the use of metabolic modeling to design defined growth media for symbiotic bacteria and may provide novel inhibitory targets to block trypanosome transmission.\n          <\/jats:p>","DOI":"10.1128\/mbio.02106-18","type":"journal-article","created":{"date-parts":[[2019,1,3]],"date-time":"2019-01-03T13:11:39Z","timestamp":1546521099000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["A Tale of Three Species: Adaptation of Sodalis glossinidius to Tsetse Biology,\n            <i>Wigglesworthia<\/i>\n            Metabolism, and Host Diet"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6701-5408","authenticated-orcid":false,"given":"Rebecca J.","family":"Hall","sequence":"first","affiliation":[{"name":"Department of Biology, University of York, York, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsey A.","family":"Flanagan","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, York, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5790-1756","authenticated-orcid":false,"given":"Michael J.","family":"Bottery","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, York, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7430-3712","authenticated-orcid":false,"given":"Vicki","family":"Springthorpe","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, York, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Thorpe","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, York, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3786-6209","authenticated-orcid":false,"given":"Alistair C.","family":"Darby","sequence":"additional","affiliation":[{"name":"University of Liverpool, Institute of Integrative Biology, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6119-852X","authenticated-orcid":false,"given":"A. 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