{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:40:38Z","timestamp":1792798838365,"version":"4.5.0"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"The United States Defense Threat Reduction Agency","award":["HDTRA1-15-1-0050"],"award-info":[{"award-number":["HDTRA1-15-1-0050"]}],"id":[{"id":"https:\/\/ror.org\/04tz64554","id-type":"ROR","asserted-by":"crossref"}]},{"name":"The National Research Foundation, Singapore","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"https:\/\/ror.org\/03cpyc314","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2021,12,21]]},"abstract":"<jats:p>Treatment for shigellosis is becoming increasingly difficult as resistance to antibiotics becomes more prevalent. One way to prevent this significant public health problem from developing into a full-blown crisis is to approach shigellosis intervention from the point of view of the host.<\/jats:p>","DOI":"10.1128\/mbio.02158-21","type":"journal-article","created":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T05:03:44Z","timestamp":1640063024000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["High-Throughput CRISPR Screens To Dissect Macrophage-\n                    <i>Shigella<\/i>\n                    Interactions"],"prefix":"10.1128","volume":"12","author":[{"given":"Yong","family":"Lai","sequence":"first","affiliation":[{"name":"Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore"},{"name":"Synthetic Biology Group, MIT Synthetic Biology Center, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA"},{"name":"Research Laboratory of Electronics, MIT, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Cui","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory H.","family":"Babunovic","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah M.","family":"Fortune","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USA"},{"name":"Ragon Institute of MGH, MIT, and Harvard, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John G.","family":"Doench","sequence":"additional","affiliation":[{"name":"Broad Institute, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3918-8923","authenticated-orcid":true,"given":"Timothy K.","family":"Lu","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore"},{"name":"Synthetic Biology Group, MIT Synthetic Biology Center, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA"},{"name":"Research Laboratory of Electronics, MIT, Cambridge, Massachusetts, USA"},{"name":"Broad Institute, Cambridge, Massachusetts, USA"},{"name":"Department of Electrical Engineering and Computer Science, MIT, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)33296-8"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30475-4"},{"key":"e_1_3_2_4_2","volume-title":"Guidelines for the control of shigellosis, including epidemics due to Shigella dysenteriae type 1","author":"World Health Organization","year":"2005","unstructured":"World Health Organization. 2005. Guidelines for the control of shigellosis, including epidemics due to Shigella dysenteriae type 1. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00344-16"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03188-20"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000475"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/20469047.2017.1409454"},{"key":"e_1_3_2_9_2","volume-title":"Antibiotic resistance threats in the United States","author":"Centers for Disease Control and Prevention","year":"2013","unstructured":"Centers for Disease Control and Prevention. 2013. Antibiotic resistance threats in the United States. CDC, Atlanta, GA."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30753-3"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.3.858-863.1989"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2010.08.014"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12367"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00219"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00038"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1902922116"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2001619"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.04.012"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2008.12.011"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.01.013"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf522"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003926"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030111"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.07.009"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006224"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.25"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.8.4379-4388.2002"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00751-19"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2019.09.007"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3437"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2020.08.010"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07901-8"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201846943"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.04.063"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0433-3"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1406694111"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01575-13"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2012.124"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1324021111"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1116302108"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9746"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3867"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(91)90128-S"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.03.001"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.10.030"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.10.028"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00079"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040248"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071990"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12534"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.59022"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1960"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature18590"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature18629"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.02.005"},{"key":"e_1_3_2_57_2","volume-title":"Ending preventable child deaths from pneumonia and diarrhoea by 2025: the integrated global action plan for pneumonia and diarrhoea (GAPPD)","author":"World Health Organization, UNICEF","year":"2013","unstructured":"World Health Organization, UNICEF. 2013. Ending preventable child deaths from pneumonia and diarrhoea by 2025: the integrated global action plan for pneumonia and diarrhoea (GAPPD). World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112738"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature24467"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.06.007"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.43.1.397-401.1984"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.18.1.94-98.1977"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.02.038"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1180"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013984"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02086-y"},{"key":"e_1_3_2_68_2","unstructured":"Lai Y Lu TK. 8 April 2020. Filed patent (WO 2021\/064655; PCT\/IB2020\/059240)."}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.02158-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.02158-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T22:52:18Z","timestamp":1646693538000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.02158-21"}},"subtitle":[],"editor":[{"given":"Sang Yup","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,12,21]]},"references-count":67,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12,21]]}},"alternative-id":["10.1128\/mBio.02158-21"],"URL":"https:\/\/doi.org\/10.1128\/mbio.02158-21","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.04.25.061671","asserted-by":"object"}]},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,21]]},"assertion":[{"value":"2021-07-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02158-21"}}