{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T08:36:08Z","timestamp":1773477368973,"version":"3.50.1"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T00:00:00Z","timestamp":1729036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T00:00:00Z","timestamp":1729036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["HHSN272201400007C"],"award-info":[{"award-number":["HHSN272201400007C"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"name":"USDA | National Institute of Food and Agriculture","award":["PEN04955"],"award-info":[{"award-number":["PEN04955"]}]},{"name":"HHS | NIH | NIAID | Division of Intramural Research"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2024,10,16]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>Split-virion-inactivated influenza vaccines are formulated based on viral hemagglutinin content. These vaccines also contain the viral neuraminidase (NA) protein, but NA content is not standardized and varies between manufacturers. In clinical studies and animal models, antibodies directed toward NA reduced disease severity and viral load; however, the impact of vaccine-induced NA immunity on airborne transmission of influenza A viruses is not well characterized. Therefore, we evaluated if vaccination against NA could disrupt chains of airborne transmission for the 2009 pandemic H1N1 virus in ferrets. Immunologically na\u00efve donor ferrets were infected with the 2009 pandemic H1N1 virus and then paired in transmission cages with mock- or NA-vaccinated respiratory contacts. The mock- and NA-vaccinated animals were then monitored daily for infection, and once infected, these animals were paired with a naive secondary respiratory contact. In these studies, all mock- and NA-vaccinated animals became infected; however, NA-vaccinated animals shed significantly less virus for fewer days relative to mock-vaccinated animals. For the secondary contacts, 6\/6 and 5\/6 animals became infected after exposure to mock- and NA-vaccinated animals, respectively. To determine if vaccine-induced immune pressure selected for escape variants, we sequenced viruses recovered from ferrets. No mutations in NA became enriched during transmission. These findings indicate that despite reducing viral load, vaccine-induced NA immunity does not prevent infection during continuous airborne exposure and subsequent onward airborne transmission of the 2009 pandemic H1N1 virus.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>In humans and animal models, immunity against neuraminidase (NA) reduces disease severity and viral replication during influenza infection. However, we have a limited understanding of the impact of NA immunity on viral transmission. Using chains of airborne transmission in ferrets as a strategy to simulate a more natural route of infection, we assessed if vaccine-induced NA immunity could disrupt transmission of the 2009 pandemic H1N1 virus. The 2009 pandemic H1N1 virus transmitted efficiently through chains of transmission in the presence of NA immunity, but NA-vaccinated animals shed significantly less virus and had accelerated viral clearance. To determine if immune pressure led to the generation of escape variants, viruses in ferret nasal wash samples were sequenced, and no mutations in NA were identified. These findings demonstrate that vaccine-induced NA immunity is not sufficient to prevent infection via airborne exposure and onward airborne transmission of the 2009 pandemic H1N1 virus.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.02161-24","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T13:02:12Z","timestamp":1725886932000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Vaccine-induced NA immunity decreases viral shedding, but does not disrupt chains of airborne transmission for the 2009 pandemic H1N1 virus in ferrets"],"prefix":"10.1128","volume":"15","author":[{"given":"K. M.","family":"Septer","sequence":"first","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania, USA"},{"name":"Huck Institutes of Life Sciences, Pennsylvania State University, University Park, Pennsylvania, USA"},{"name":"Emory-UGA Center of Excellence of Influenza Research and Response (CEIRR), University Park, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. A.","family":"Heinly","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania, USA"},{"name":"Huck Institutes of Life Sciences, Pennsylvania State University, University Park, Pennsylvania, USA"},{"name":"Emory-UGA Center of Excellence of Influenza Research and Response (CEIRR), University Park, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. G.","family":"Sim","sequence":"additional","affiliation":[{"name":"Huck Institutes of Life Sciences, Pennsylvania State University, University Park, Pennsylvania, USA"},{"name":"Department of Biology, Pennsylvania State University, University Park, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. R.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania, USA"},{"name":"Huck Institutes of Life Sciences, Pennsylvania State University, University Park, Pennsylvania, USA"},{"name":"Emory-UGA Center of Excellence of Influenza Research and Response (CEIRR), University Park, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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