{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:06:14Z","timestamp":1788570374862,"version":"build-2803163510"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Fi 459\/22-1 and Fi 459\/19-2"],"award-info":[{"award-number":["Fi 459\/22-1 and Fi 459\/19-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC 31861133014"],"award-info":[{"award-number":["NSFC 31861133014"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC W2411075"],"award-info":[{"award-number":["NSFC W2411075"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC 82172292"],"award-info":[{"award-number":["NSFC 82172292"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,11,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Phytochromes are red-light receptors that regulate gene expression in response to light and temperature in plants, bacteria, and fungi. In the opportunistic pathogenic fungus\n                      <jats:italic toggle=\"yes\">Aspergillus fumigatus<\/jats:italic>\n                      , two putative phytochromes\u2014FphA and FphB\u2014were characterized to assess any roles in virulence. Spectroscopic analysis of\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      -expressed proteins showed that FphA is photoconvertible and functionally similar to its\n                      <jats:italic toggle=\"yes\">A. nidulans<\/jats:italic>\n                      ortholog, whereas FphB is photoinactive. Therefore, only FphA could rescue an\n                      <jats:italic toggle=\"yes\">A. nidulans fphA<\/jats:italic>\n                      -deletion mutant. Gene deletion in\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      revealed that loss of FphA had no effect on virulence, while deletion of FphB\u2014alone or with FphA\u2014significantly increased virulence in a\n                      <jats:italic toggle=\"yes\">Galleria mellonella<\/jats:italic>\n                      infection model. Transcriptomic data linked FphB to the regulation of the neosartoricin B biosynthetic gene cluster and the production of the prenylated nonribosomal peptide hexadehydroastechrome, suggesting a role in regulating secondary metabolism. Localization studies showed FphA at the mitochondria and in nuclei and FphB mainly in the cytoplasm; both proteins form heterodimers in the cytoplasm and in nuclei. These findings suggest that while FphA acts as a light and temperature sensor, FphB modulates virulence and may represent a novel regulatory factor in fungal pathogenicity.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Aspergillus fumigatus<\/jats:italic>\n                        is a major pathogen in immunocompromised individuals, showing greater virulence than\n                        <jats:italic toggle=\"yes\">A. nidulans<\/jats:italic>\n                        despite genetic similarities. A key difference is the presence of two phytochrome-like proteins: FphA, a conserved red-light and temperature sensor, and FphB, a photoinactive hybrid histidine kinase. Notably, FphB appears to suppress virulence, suggesting a regulatory role in signaling pathways that govern pathogenicity and secondary metabolism. We propose FphB functions as a signaling hub linking environmental cues to virulence, with its network offering a promising target for antifungal strategies.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.02204-25","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:00:27Z","timestamp":1758632427000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Distinct roles of phytochromes A and B in\n                    <i>Aspergillus fumigatus<\/i>\n                    in environmental sensing and pathogenicity"],"prefix":"10.1128","volume":"16","author":[{"given":"Kai","family":"Leister","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04t3en479","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Institute for Applied Biosciences, Karlsruhe Institute of Technology (KIT)-South Campus","place":["Karlsruhe, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3837-9086","authenticated-orcid":false,"given":"Yan","family":"Dong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036trcv74","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Nanjing Drum Tower Hospital, College of Life Sciences, Nanjing Normal University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Landmark","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04t3en479","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Institute for Applied Biosciences, Karlsruhe Institute of Technology (KIT)-South Campus","place":["Karlsruhe, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinyan","family":"Ma","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036trcv74","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Nanjing Drum Tower Hospital, College of Life Sciences, Nanjing Normal University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Schreckenberger","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04t3en479","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Institute for Applied Biosciences, Karlsruhe Institute of Technology (KIT)-South Campus","place":["Karlsruhe, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhong","family":"Yu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2891-7326","authenticated-orcid":true,"given":"Ling","family":"Lu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036trcv74","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Nanjing Drum Tower Hospital, College of Life Sciences, Nanjing Normal University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6704-2569","authenticated-orcid":true,"given":"Reinhard","family":"Fischer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04t3en479","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Institute for Applied Biosciences, Karlsruhe Institute of Technology (KIT)-South Campus","place":["Karlsruhe, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0109-x"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.112408.134000"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2020.01.004"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/414776a"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.08.061"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00142-13"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.12.2140-2152.2005"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009845"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-120417-031415"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00371-19"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805506200"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2021108083"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.01.061"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.19"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13062"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14395"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf5656"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf6005"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01517-16"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-013-0387-9"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12911"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2024.107238"},{"key":"e_1_3_4_24_2","volume-title":"Molecular characterization of the light response in Alternaria alternata","author":"Igbalajobi OA","year":"2019","unstructured":"Igbalajobi OA. 2019. Molecular characterization of the light response in Alternaria alternata. KIT, Karlsruhe."},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201300070"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol303435y"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja311145n"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E10-11-0914"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13007"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-004-0495-7"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.70292"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-008-0390-x"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00285.x"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1155888"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13275"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12032"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00222-12"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077147"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009235"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.5.1470"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0065-2660(08)60408-3"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.4148\/1941-4765.1173"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05257.x"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.5.1.207-211.2006"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02501"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(86)90173-9"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.02204-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.02204-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T14:04:40Z","timestamp":1762956280000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mbio.02204-25"}},"subtitle":[],"editor":[{"given":"Alexander","family":"Idnurm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,11,12]]},"references-count":46,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11,12]]}},"alternative-id":["10.1128\/mbio.02204-25"],"URL":"https:\/\/doi.org\/10.1128\/mbio.02204-25","relation":{},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,12]]},"assertion":[{"value":"2025-07-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02204-25"}}