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In this study, we demonstrated that\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            can grow efficiently on intestinal mucus and its component sialic acids and that a tripartite ATP-independent periplasmic SiaPQM strain, transporter-deficient mutant NC1777, was attenuated for colonization using a streptomycin-pretreated adult mouse model. In\n            <jats:italic>in vivo<\/jats:italic>\n            competition assays, NC1777 was significantly outcompeted for up to 3\u00a0days postinfection. NC1777 was also significantly outcompeted in\n            <jats:italic>in vitro<\/jats:italic>\n            competition assays in M9 minimal medium supplemented with intestinal mucus, indicating that sialic acid uptake is essential for fitness. Phylogenetic analyses demonstrated that the ability to utilize sialic acid was distributed among 452 bacterial species from eight phyla. The majority of species belonged to four phyla,\n            <jats:italic>Actinobacteria<\/jats:italic>\n            (members of\n            <jats:italic>Actinobacillus<\/jats:italic>\n            ,\n            <jats:italic>Corynebacterium<\/jats:italic>\n            ,\n            <jats:italic>Mycoplasma<\/jats:italic>\n            , and\n            <jats:italic>Streptomyces<\/jats:italic>\n            ),\n            <jats:italic>Bacteroidetes<\/jats:italic>\n            (mainly\n            <jats:italic>Bacteroides<\/jats:italic>\n            ,\n            <jats:italic>Capnocytophaga<\/jats:italic>\n            , and\n            <jats:italic>Prevotella<\/jats:italic>\n            ),\n            <jats:italic>Firmicutes<\/jats:italic>\n            (members of\n            <jats:italic>Streptococcus<\/jats:italic>\n            ,\n            <jats:italic>Staphylococcus<\/jats:italic>\n            ,\n            <jats:italic>Clostridium<\/jats:italic>\n            , and\n            <jats:italic>Lactobacillus<\/jats:italic>\n            ), and\n            <jats:italic>Proteobacteria<\/jats:italic>\n            (including\n            <jats:italic>Escherichia<\/jats:italic>\n            ,\n            <jats:italic>Shigella<\/jats:italic>\n            ,\n            <jats:italic>Salmonella<\/jats:italic>\n            ,\n            <jats:italic>Citrobacter<\/jats:italic>\n            ,\n            <jats:italic>Haemophilus<\/jats:italic>\n            ,\n            <jats:italic>Klebsiella<\/jats:italic>\n            ,\n            <jats:italic>Pasteurella<\/jats:italic>\n            ,\n            <jats:italic>Photobacterium<\/jats:italic>\n            ,\n            <jats:italic>Vibrio<\/jats:italic>\n            , and\n            <jats:italic>Yersinia<\/jats:italic>\n            species), mostly commensals and\/or pathogens. Overall, our data demonstrate that the ability to take up host-derived sugars and sialic acid specifically allows\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            a competitive advantage in intestinal colonization and that this is a trait that is sporadic in its occurrence and phylogenetic distribution and ancestral in some genera but horizontally acquired in others.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Sialic acids are nine carbon amino sugars that are abundant on all mucous surfaces. The deadly human pathogen\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            contains the genes required for scavenging, transport, and catabolism of sialic acid. We determined that the\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            SiaPQM transporter is essential for sialic acid transport and that this trait allows the bacterium to outcompete noncatabolizers\n            <jats:italic>in vivo<\/jats:italic>\n            . We also showed that the ability to take up and catabolize sialic acid is prevalent among both commensals and pathogens that colonize the oral cavity and the respiratory, intestinal, and urogenital tracts. Phylogenetic analysis determined that the sialic acid catabolism phenotype is ancestral in some genera such as\n            <jats:italic>Yersinia<\/jats:italic>\n            ,\n            <jats:italic>Streptococcus<\/jats:italic>\n            , and\n            <jats:italic>Staphylococcus<\/jats:italic>\n            and is acquired by horizontal gene transfer in others such as\n            <jats:italic>Vibrio<\/jats:italic>\n            ,\n            <jats:italic>Aeromonas<\/jats:italic>\n            , and\n            <jats:italic>Klebsiella<\/jats:italic>\n            . The data demonstrate that this trait has evolved multiple times in different lineages, indicating the importance of specialized metabolism to niche expansion.\n          <\/jats:p>","DOI":"10.1128\/mbio.02237-15","type":"journal-article","created":{"date-parts":[[2016,4,13]],"date-time":"2016-04-13T01:29:52Z","timestamp":1460510992000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":69,"title":["Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness\n            <i>In Vivo<\/i>"],"prefix":"10.1128","volume":"7","author":[{"given":"Nathan D.","family":"McDonald","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Delaware, Newark, Delaware, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Bernard","family":"Lubin","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Delaware, Newark, Delaware, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nityananda","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Delaware, Newark, Delaware, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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