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Most isolates from African patients with bacteremia belong to a single sequence type, ST313, which is genetically distinct from gastroenteritis-associated ST19 strains, such as 14028s and SL1344. Some studies suggest that the rapid spread of ST313 across sub-Saharan Africa has been facilitated by anthroponotic (person-to-person) transmission, eliminating the need for\n            <jats:italic>Salmonella<\/jats:italic>\n            survival outside the host. While these studies have not ruled out zoonotic or other means of transmission, the anthroponotic hypothesis is supported by evidence of extensive genomic decay, a hallmark of host adaptation, in the sequenced ST313 strain D23580. We have identified and demonstrated 2 loss-of-function mutations in D23580, not present in the ST19 strain 14028s, that impair multicellular stress resistance associated with survival outside the host. These mutations result in inactivation of the KatE stationary-phase catalase that protects high-density bacterial communities from oxidative stress and the BcsG cellulose biosynthetic enzyme required for the RDAR (red, dry, and rough) colonial phenotype. However, we found that like 14028s, D23580 is able to elicit an acute inflammatory response and cause enteritis in mice and rhesus macaque monkeys. Collectively, these observations suggest that African\n            <jats:italic>S<\/jats:italic>\n            .\u00a0Typhimurium ST313 strain D23580 is becoming adapted to an anthroponotic mode of transmission while retaining the ability to infect and cause enteritis in multiple host species.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            The last 3 decades have witnessed an epidemic of invasive nontyphoidal\n            <jats:italic>Salmonella<\/jats:italic>\n            infections in sub-Saharan Africa. Genomic analysis and clinical observations suggest that the\n            <jats:italic>Salmonella<\/jats:italic>\n            strains responsible for these infections are evolving to become more typhoid-like with regard to patterns of transmission and virulence. This study shows that a prototypical African nontyphoidal\n            <jats:italic>Salmonella<\/jats:italic>\n            strain has lost traits required for environmental stress resistance, consistent with an adaptation to a human-to-human mode of transmission. However, in contrast to predictions, the strain remains capable of causing acute inflammation in the mammalian intestine. This suggests that the systemic clinical presentation of invasive nontyphoidal\n            <jats:italic>Salmonella<\/jats:italic>\n            infections in Africa reflects the immune status of infected hosts rather than intrinsic differences in the virulence of African\n            <jats:italic>Salmonella<\/jats:italic>\n            strains. Our study provides important new insights into the evolution of host adaptation in bacterial pathogens.\n          <\/jats:p>","DOI":"10.1128\/mbio.02265-15","type":"journal-article","created":{"date-parts":[[2016,3,2]],"date-time":"2016-03-02T03:03:54Z","timestamp":1456887834000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["Loss of Multicellular Behavior in Epidemic African Nontyphoidal Salmonella enterica Serovar Typhimurium ST313 Strain D23580"],"prefix":"10.1128","volume":"7","author":[{"given":"Larissa A.","family":"Singletary","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyce E.","family":"Karlinsey","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Libby","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, School of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason P.","family":"Mooney","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen L.","family":"Lokken","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9e M.","family":"Tsolis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and 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