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Posttranslationally modified microtubules encase the inclusion, controlling the positioning of Golgi complex fragments around the inclusion. The molecular mechanisms by which\n            <jats:italic>Chlamydia<\/jats:italic>\n            coopts the host cytoskeleton and the Golgi complex to sustain its infectious compartment are unknown. Here, using a genetically modified\n            <jats:italic>Chlamydia<\/jats:italic>\n            strain, we discovered that both posttranslationally modified microtubules and Golgi complex positioning around the inclusion are controlled by the chlamydial inclusion protein CT813\/CTL0184\/InaC and host ARF GTPases. CT813 recruits ARF1 and ARF4 to the inclusion membrane, where they induce posttranslationally modified microtubules. Similarly, both ARF isoforms are required for the repositioning of Golgi complex fragments around the inclusion. We demonstrate that CT813 directly recruits ARF GTPases on the inclusion membrane and plays a pivotal role in their activation. Together, these results reveal that\n            <jats:italic>Chlamydia<\/jats:italic>\n            uses CT813 to hijack ARF GTPases to couple posttranslationally modified microtubules and Golgi complex repositioning at the inclusion.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            is an important cause of morbidity and a significant economic burden in the world. However, how\n            <jats:italic>Chlamydia<\/jats:italic>\n            develops its intracellular compartment, the so-called inclusion, is poorly understood. Using genetically engineered\n            <jats:italic>Chlamydia<\/jats:italic>\n            mutants, we discovered that the effector protein CT813 recruits and activates host ADP-ribosylation factor 1 (ARF1) and ARF4 to regulate microtubules. In this context, CT813 acts as a molecular platform that induces the posttranslational modification of microtubules around the inclusion. These cages are then used to reposition the Golgi complex during infection and promote the development of the inclusion. This study provides the first evidence that ARF1 and ARF4 play critical roles in controlling posttranslationally modified microtubules around the inclusion and that\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            hijacks this novel function of ARF to reposition the Golgi complex.\n          <\/jats:p>","DOI":"10.1128\/mbio.02280-16","type":"journal-article","created":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T00:10:45Z","timestamp":1493770245000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["<i>Chlamydia<\/i>\n            Hijacks ARF GTPases To Coordinate Microtubule Posttranslational Modifications and Golgi Complex Positioning"],"prefix":"10.1128","volume":"8","author":[{"given":"Jordan","family":"Wesolowski","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary M.","family":"Weber","sequence":"additional","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Nawrotek","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie and Pharmacologie Appliqu\u00e9e, Centre National de la Recherche Scientifique, Ecole Normale Sup\u00e9rieure Paris-Saclay, Cachan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl A.","family":"Dooley","sequence":"additional","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Calderon","sequence":"additional","affiliation":[{"name":"Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudette M.","family":"St. Croix","sequence":"additional","affiliation":[{"name":"Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ted","family":"Hackstadt","sequence":"additional","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Cherfils","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie and Pharmacologie 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