{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T02:46:51Z","timestamp":1781318811574,"version":"3.54.1"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2015,2,17]],"date-time":"2015-02-17T00:00:00Z","timestamp":1424131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T00:00:00Z","timestamp":1424995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2015,2,27]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Inhalation of\n            <jats:named-content content-type=\"genus-species\">Yersinia pestis<\/jats:named-content>\n            results in primary pneumonic plague, a highly lethal and rapidly progressing necrotizing pneumonia. The disease begins with a period of extensive bacterial replication in the absence of disease symptoms, followed by the sudden onset of inflammatory responses that ultimately prove fatal. Very little is known about the bacterial and host factors that contribute to the rapid biphasic progression of pneumonic plague. In this work, we analyzed the\n            <jats:italic>in vivo<\/jats:italic>\n            transcription kinetics of 288 bacterial open reading frames previously shown by microarray analysis to be dynamically regulated in the lung. Using this approach combined with bacterial genetics, we were able to identify five Y.\u00a0pestis genes that contribute to the development of pneumonic plague. Deletion of one of these genes,\n            <jats:italic>ybtX<\/jats:italic>\n            , did not alter bacterial survival but attenuated host inflammatory responses during late-stage disease. Deletion of\n            <jats:italic>ybtX<\/jats:italic>\n            in another lethal respiratory pathogen,\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            , also resulted in diminished host inflammation during infection. Thus, our\n            <jats:italic>in vivo<\/jats:italic>\n            transcriptional screen has identified an important inflammatory mediator that is common to two Gram-negative bacterial pathogens that cause severe pneumonia.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Yersinia\u00a0pestis is responsible for at least three major pandemics, most notably the Black Death of the Middle Ages. Due to its pandemic potential, ease of dissemination by aerosolization, and a history of its weaponization, Y.\u00a0pestis is categorized by the Centers for Disease Control and Prevention as a tier 1 select agent most likely to be used as a biological weapon. To date, there is no licensed vaccine against Y.\u00a0pestis. Importantly, an early \u201csilent\u201d phase followed by the rapid onset of nondescript influenza-like symptoms makes timely treatment of pneumonic plague difficult. A more detailed understanding of the bacterial and host factors that contribute to pathogenesis is essential to understanding the progression of pneumonic plague and developing or enhancing treatment options.\n          <\/jats:p>","DOI":"10.1128\/mbio.02302-14","type":"journal-article","created":{"date-parts":[[2015,2,18]],"date-time":"2015-02-18T07:20:41Z","timestamp":1424244041000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["<i>In Vivo<\/i>\n            Transcriptional Profiling of Yersinia pestis Reveals a Novel Bacterial Mediator of Pulmonary Inflammation"],"prefix":"10.1128","volume":"6","author":[{"given":"Roger D.","family":"Pechous","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher A.","family":"Broberg","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolas M.","family":"Stasulli","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia L.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1551-6718","authenticated-orcid":false,"given":"William E.","family":"Goldman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.283.17.2281"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.11.009"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/017335-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00403-06"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506840102"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.04.008"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01348.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01236-09"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12693"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.2741\/2853"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00699.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003303"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2800"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2010.21"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003679"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","unstructured":"Di Martino P Bertin Y Girardeau JP Livrelli V Joly B Darfeuille-Michaud A. 1995. Molecular characterization and adhesive properties of CF29K an adhesin of Klebsiella pneumoniae strains involved in nosocomial infections. Infect Immun 63:4336\u20134344.","DOI":"10.1128\/iai.63.11.4336-4344.1995"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.6.2701-2706.2004"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.12703\/P6-64"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00372-06"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01412-5"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04694.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02235.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003388"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.029611-0"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-009-9286-4"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.037945-0"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(98)90115-6"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-5-1115"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00368.2009"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0b013e3283312956"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094934"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00280.2006"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.01.009"},{"key":"e_1_3_2_35_2","unstructured":"Mont\u00f3n C Torres A. 1998. Lung inflammatory response in pneumonia. Monaldi Arch Chest Dis 53:56\u201363."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/368339a0"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(94)80014-6"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1186\/cc8155"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2007.10.009"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2010.10.002"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7304-7310.2005"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.26.021607.090326"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5208"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003324"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01296-09"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2012.00155"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir696"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1137195"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth765"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00793-8"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_2_53_2","volume-title":"User bulletin #2. ABI Prism 7700 Sequence Detection System","author":"Applied Biosystems","year":"1997","unstructured":"Applied Biosystems. 1997. User bulletin #2. ABI Prism 7700 Sequence Detection System. Applied Biosystems, Foster City, CA."}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.02302-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.02302-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T02:03:53Z","timestamp":1646705033000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.02302-14"}},"subtitle":[],"editor":[{"given":"Jeff F.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,2,27]]},"references-count":52,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,2,27]]}},"alternative-id":["10.1128\/mBio.02302-14"],"URL":"https:\/\/doi.org\/10.1128\/mbio.02302-14","relation":{},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,27]]},"assertion":[{"value":"2014-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02302-14"}}