{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:09:09Z","timestamp":1790629749358,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2014,12,30]],"date-time":"2014-12-30T00:00:00Z","timestamp":1419897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"},{"start":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T00:00:00Z","timestamp":1424995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2015,2,27]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In a screen for compounds that inhibit infectivity of the obligate intracellular pathogen\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            , we identified the 2-pyridone amide KSK120. A fluorescent KSK120 analogue was synthesized and observed to be associated with the C.\u00a0trachomatis surface, suggesting that its target is bacterial. We isolated KSK120-resistant strains and determined that several resistance mutations are in genes that affect the uptake and use of glucose-6-phosphate (G-6P). Consistent with an effect on G-6P metabolism, treatment with KSK120 blocked glycogen accumulation. Interestingly, KSK120 did not affect\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            or the host cell. Thus, 2-pyridone amides may represent a class of drugs that can specifically inhibit C.\u00a0trachomatis infection.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            is a bacterial pathogen of humans that causes a common sexually transmitted disease as well as eye infections. It grows only inside cells of its host organism, within a parasitophorous vacuole termed the inclusion. Little is known, however, about what bacterial components and processes are important for C.\u00a0trachomatis cellular infectivity. Here, by using a visual screen for compounds that affect bacterial distribution within the chlamydial inclusion, we identified the inhibitor KSK120. As hypothesized, the altered bacterial distribution induced by KSK120 correlated with a block in C.\u00a0trachomatis infectivity. Our data suggest that the compound targets the glucose-6-phosphate (G-6P) metabolism pathway of C.\u00a0trachomatis, supporting previous indications that G-6P metabolism is critical for C.\u00a0trachomatis infectivity. Thus, KSK120 may be a useful tool to study chlamydial glucose metabolism and has the potential to be used in the treatment of C.\u00a0trachomatis infections.\n          <\/jats:p>","DOI":"10.1128\/mbio.02304-14","type":"journal-article","created":{"date-parts":[[2014,12,31]],"date-time":"2014-12-31T03:33:59Z","timestamp":1419996839000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["A 2-Pyridone-Amide Inhibitor Targets the Glucose Metabolism Pathway of Chlamydia trachomatis"],"prefix":"10.1128","volume":"6","author":[{"given":"Patrik","family":"Engstr\u00f6m","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Ume\u00e5 University, Ume\u00e5, Sweden"},{"name":"Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume\u00e5 University, Ume\u00e5, Sweden"},{"name":"Ume\u00e5 Centre for Microbial Research (UCMR), Ume\u00e5 University, Ume\u00e5, Sweden"},{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Syam","family":"Krishnan","sequence":"additional","affiliation":[{"name":"Ume\u00e5 Centre for Microbial Research (UCMR), Ume\u00e5 University, Ume\u00e5, Sweden"},{"name":"Department of Chemistry, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bidong D.","family":"Ngyuen","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Center for Microbial Pathogenesis, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Chorell","sequence":"additional","affiliation":[{"name":"Ume\u00e5 Centre for Microbial Research (UCMR), Ume\u00e5 University, Ume\u00e5, Sweden"},{"name":"Department of Chemistry, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Normark","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, 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