{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T21:51:13Z","timestamp":1783547473697,"version":"3.55.0"},"reference-count":75,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T00:00:00Z","timestamp":1702944000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T00:00:00Z","timestamp":1702944000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01AI153332"],"award-info":[{"award-number":["R01AI153332"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["R01NS116716"],"award-info":[{"award-number":["R01NS116716"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["F31AI164674-01"],"award-info":[{"award-number":["F31AI164674-01"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2023,12,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Group B\n            <jats:italic>Streptococcus<\/jats:italic>\n            (GBS) colonizes the female reproductive tract (FRT) and causes adverse pregnancy outcomes and invasive disease following vertical transmission to the fetus or newborn. Despite this major public health burden, the mechanisms of GBS FRT colonization are understudied. A recent transposon sequencing screen identified GBS factors contributing to vaginal colonization and ascending spread, including a putative DNA-cytosine methyltransferase (Dcm). We constructed a \u0394\n            <jats:italic>dcm<\/jats:italic>\n            deletion strain and confirmed that\n            <jats:italic>dcm<\/jats:italic>\n            contributes to murine FRT colonization. Investigation of the evolutionary origin of the\n            <jats:italic>dcm<\/jats:italic>\n            gene reveals that it is widely distributed across GBS and is encoded as part of a prophage genome that displays evidence of horizontal transfer between GBS strains. We further show that Dcm contributes to 5mC methylation and global regulation of genes involved in carbohydrate metabolism, transcription regulation, and known adhesins and metabolic factors involved in GBS colonization. Interestingly, GBS genes that are induced in the presence of the highly glycosylated vaginal mucin MUC5B were significantly downregulated in the \u2206\n            <jats:italic>dcm<\/jats:italic>\n            mutant. Furthermore, the \u2206\n            <jats:italic>dcm<\/jats:italic>\n            mutant exhibited reduced binding to immobilized mucin and was attenuated in its ability to grow on numerous carbon sources including the carbohydrates found on mucins. While the \u2206\n            <jats:italic>dcm<\/jats:italic>\n            mutant displayed enhanced clearance from the FRT in wild-type mice, there was no significant difference in\n            <jats:italic>MUC5B<\/jats:italic>\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice, indicating that Dcm-mediated regulation requires MUC5B to promote GBS colonization. This is the first report to characterize the impact of a DNA methyltransferase on GBS gene regulation and FRT colonization.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Group B\n              <jats:italic>Streptococcus<\/jats:italic>\n              (GBS) colonizes the female reproductive tract (FRT) in one-third of women, and carriage leads to numerous adverse pregnancy outcomes including the preterm premature rupture of membranes, chorioamnionitis, and stillbirth. The presence of GBS in the FRT during pregnancy is also the largest predisposing factor for the transmission of GBS and invasive neonatal diseases, including pneumonia, sepsis, and meningitis. The factors contributing to GBS colonization are still being elucidated. Here, we show for the first time that GBS transcription is regulated by an orphan DNA cytosine methyltransferase (Dcm). Many GBS factors are regulated by Dcm, especially those involved in carbohydrate transport and metabolism. We show that GBS persistence in the FRT is dependent on the catabolism of sugars found on the vaginal mucin MUC5B. Collectively, this work highlights the regulatory importance of a DNA methyltransferase and identifies both host and bacterial factors required for GBS colonization.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.02306-23","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T13:05:05Z","timestamp":1698757505000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Identification of a DNA-cytosine methyltransferase that impacts global transcription to promote group B streptococcal vaginal colonization"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2864-6212","authenticated-orcid":false,"given":"Haider S.","family":"Manzer","sequence":"first","affiliation":[{"name":"Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonya","family":"Brunetti","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4232-6837","authenticated-orcid":true,"given":"Kelly S.","family":"Doran","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0029-7844(00)00977-7"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.JPN.0000333925.30328.fd"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix664"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix585"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.14734\/PN.2018.29.4.147"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/314756"},{"key":"e_1_3_6_8_2","first-page":"816","article-title":"Peripartum infection associated with vaginal group B streptococcal colonization","volume":"84","author":"Yancey MK","year":"1994","unstructured":"Yancey MK, Duff P, Clark P, Kurtzer T, Frentzen BH, Kubilis P. 1994. 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