{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:21:44Z","timestamp":1790014904625,"version":"4.0.1"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T00:00:00Z","timestamp":1577145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000690","name":"RCUK","doi-asserted-by":"crossref","award":["MR\/M026434\/1"],"award-info":[{"award-number":["MR\/M026434\/1"]}],"id":[{"id":"10.13039\/501100000690","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["310155\/2017-7"],"award-info":[{"award-number":["310155\/2017-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["313633\/2017-7"],"award-info":[{"award-number":["313633\/2017-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"crossref","award":["BB\/L022869\/1"],"award-info":[{"award-number":["BB\/L022869\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"name":"MRC","award":["MR\/K01224X\/1"],"award-info":[{"award-number":["MR\/K01224X\/1"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2019,12,24]]},"abstract":"<jats:p>\n            Leprosy remains a major problem in the world today, particularly affecting the poorest and most disadvantaged sections of society in the least developed countries of the world. The long-term aim of research is to develop new treatments and vaccines, and these aims are currently hampered by our inability to grow the pathogen in axenic culture. In this study, we probed the metabolism of\n            <jats:named-content content-type=\"genus-species\">M. leprae<\/jats:named-content>\n            while it is surviving and replicating inside its primary host cell, the Schwann cell, and compared it to a related pathogen,\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            , replicating in macrophages. Our analysis revealed that unlike\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">M. leprae<\/jats:named-content>\n            utilized host glucose as a carbon source and that it biosynthesized its own amino acids, rather than importing them from its host cell. We demonstrated that the enzyme phosphoenolpyruvate carboxylase plays a crucial role in glucose catabolism in\n            <jats:named-content content-type=\"genus-species\">M. leprae<\/jats:named-content>\n            . Our findings provide the first metabolic signature of\n            <jats:named-content content-type=\"genus-species\">M. leprae<\/jats:named-content>\n            in the host Schwann cell and identify novel avenues for the development of antileprosy drugs.\n          <\/jats:p>","DOI":"10.1128\/mbio.02351-19","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T12:54:47Z","timestamp":1576500887000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Intracellular Mycobacterium leprae Utilizes Host Glucose as a Carbon Source in Schwann Cells"],"prefix":"10.1128","volume":"10","author":[{"given":"Khushboo","family":"Borah","sequence":"first","affiliation":[{"name":"Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina do Carmo de Vasconcelos","family":"Girardi","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Celular, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom A.","family":"Mendum","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia Miranda Santos","family":"Lery","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Celular, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dany J. V.","family":"Beste","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio Alves","family":"Lara","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Celular, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina Vidal","family":"Pessolani","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Celular, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johnjoe","family":"McFadden","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"405","article-title":"Global leprosy update, 2015: time for action, accountability and inclusion","volume":"91","author":"World Health Organization","year":"2015","unstructured":"World Health Organization. 2015. 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