{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:22:18Z","timestamp":1790079738026,"version":"4.0.1"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T00:00:00Z","timestamp":1546905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T00:00:00Z","timestamp":1551139200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01-AI080621"],"award-info":[{"award-number":["R01-AI080621"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["F31-AI104170"],"award-info":[{"award-number":["F31-AI104170"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2019,2,26]]},"abstract":"<jats:p>\n            Inflammasomes are major components of the innate immune system and are responsible for detecting various microbial and environmental danger signals. Upon invasion of Lewis rat macrophages, the parasite rapidly activates the NLRP1 inflammasome, resulting in pyroptosis and elimination of the parasite\u2019s replication niche. The work reported here revealed that\n            <jats:italic>Toxoplasma<\/jats:italic>\n            GRA35, GRA42, and GRA43 are required for induction of Lewis rat macrophage pyroptosis. GRA42 and GRA43 mediate the correct localization of other GRAs, including GRA35, to the parasitophorous vacuole membrane. These three GRAs were also found to be important for parasite\n            <jats:italic>in vivo<\/jats:italic>\n            fitness in a\n            <jats:italic>Toxoplasma<\/jats:italic>\n            -susceptible rat strain, independently of their role in NLRP1 inflammasome activation, suggesting that they perform other important functions. Thus, this study identified three GRAs that mediate the induction of Lewis rat macrophage pyroptosis and are required for pathogenesis of the parasite.\n          <\/jats:p>","DOI":"10.1128\/mbio.02388-18","type":"journal-article","created":{"date-parts":[[2019,1,7]],"date-time":"2019-01-07T22:31:10Z","timestamp":1546900270000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Three\n            <i>Toxoplasma gondii<\/i>\n            Dense Granule Proteins Are Required for Induction of Lewis Rat Macrophage Pyroptosis"],"prefix":"10.1128","volume":"10","author":[{"given":"Yifan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly M.","family":"Cirelli","sequence":"additional","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricio D. C.","family":"Barros","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lamba Omar","family":"Sangar\u00e9","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Butty","sequence":"additional","affiliation":[{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Musa A.","family":"Hassan","sequence":"additional","affiliation":[{"name":"College of Medicine and Veterinary Medicine, The University of Edinburgh, Edinburgh, United Kingdom"},{"name":"The Roslin Institute, The University of Edinburgh, Edinburgh, United Kingdom"},{"name":"Center for Tropical Livestock Health and Genetics, The University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Pesavento","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asli","family":"Mete","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen P. J.","family":"Saeij","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2002.00485.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.6990-6997.2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506643103"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003927"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01170-13"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature15541"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature15514"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1960"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.04.015"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0403164"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01417.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.09.006"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000906"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002638"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003452"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2229"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2017.12.013"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01117-13"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1700245"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.111344"},{"key":"e_1_3_2_23_2","first-page":"114","article-title":"Sequential protein secretion from three distinct organelles of Toxoplasma gondii accompanies invasion of human fibroblasts","volume":"73","author":"Carruthers VB","year":"1997","unstructured":"Carruthers VB, Sibley LD. 1997. Sequential protein secretion from three distinct organelles of Toxoplasma gondii accompanies invasion of human fibroblasts. Eur J Cell Biol 73:114\u2013123.","journal-title":"Eur J Cell Biol"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00005-17"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.10809"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005211"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12498"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02231-15"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4005-4011.2000"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1117047109"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00808-16"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.04.003"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.019"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.8.1787-1792.1989"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20131272"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1313501111"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100717"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006052"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","unstructured":"Sandstrom A Mitchell PS Goers L Mu EW Lesser CF Vance RE. 2018. Functional degradation: a mechanism of NLRP1 inflammasome activation by diverse pathogen enzymes. bioRxiv doi:10.1101\/317834.","DOI":"10.1101\/317834"},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","unstructured":"Bachovchin D Chui AJ Okondo M Rao S Gai K Griswold A Vittimberga B. 2018. N-terminal degradation activates the Nlrp1b inflammasome. bioRxiv doi:10.1101\/317826.","DOI":"10.1101\/317826"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201600062"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10394"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10510"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2016.09.002"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01003-12"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02167-14"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.04.002"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01509.x"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100450"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005434"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg217"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.02388-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.02388-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T02:22:52Z","timestamp":1646706172000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.02388-18"}},"subtitle":[],"editor":[{"given":"Melissa","family":"Bruckner Lodoen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,2,26]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,2,26]]}},"alternative-id":["10.1128\/mBio.02388-18"],"URL":"https:\/\/doi.org\/10.1128\/mbio.02388-18","relation":{},"ISSN":["2161-2129","2150-7511"],"issn-type":[{"value":"2161-2129","type":"print"},{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,26]]},"assertion":[{"value":"2018-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02388-18"}}