{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:43:13Z","timestamp":1790538193012,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T00:00:00Z","timestamp":1603152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T00:00:00Z","timestamp":1603756800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100005847","name":"FHB | Health and Medical Research Fund","doi-asserted-by":"publisher","award":["COVID-190120"],"award-info":[{"award-number":["COVID-190120"]}],"id":[{"id":"10.13039\/501100005847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2020,10,27]]},"abstract":"<jats:p>Current commercially available serological tests for COVID-19 patients are detecting antibodies against SARS-CoV-2 nucleoprotein and spike glycoprotein. The antinucleoprotein and antispike antibodies can be accurately detected in patients during the mid or late stage of infection, and therefore, these assays have not been widely used for early diagnosis of COVID-19. In this study, we characterized the secretory property of a SARS-CoV-2 orf8 protein and proposed that orf8 secretion during infection facilitates early mounting of the B cell response. We demonstrated the presence of anti-orf8 antibodies in both symptomatic and asymptomatic patients during the early stage of infection, while the anti-N antibody is not detected. Our serological test detecting anti-orf8 antibodies may facilitate the development of early and accurate diagnosis for COVID-19.<\/jats:p>","DOI":"10.1128\/mbio.02431-20","type":"journal-article","created":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T15:39:15Z","timestamp":1603121955000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["Accurate Diagnosis of COVID-19 by a Novel Immunogenic Secreted SARS-CoV-2 orf8 Protein"],"prefix":"10.1128","volume":"11","author":[{"given":"Xiaohui","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy-Yan","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Man","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Kit","family":"Yuen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Piao","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon Wing-Ngor","family":"Au","sequence":"additional","affiliation":[{"name":"School of Life Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasper Fuk-Woo","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China"},{"name":"State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Hong Kong SAR, China"},{"name":"Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong SAR, China"},{"name":"Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1921-5824","authenticated-orcid":false,"given":"Kelvin K. 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