{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:14:06Z","timestamp":1785716046354,"version":"3.56.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003668","name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries","doi-asserted-by":"crossref","award":["321036054HD020"],"award-info":[{"award-number":["321036054HD020"]}],"id":[{"id":"10.13039\/501100003668","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["2022R1A2C109178313"],"award-info":[{"award-number":["2022R1A2C109178313"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,4,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Bacillus cereus,<\/jats:italic>\n              a Gram-positive aerobic bacterium commonly found in soil, food, and water, forms endospores that can withstand harsh environmental conditions. The endospores are encased in a protective spore coat consisting of multiple layers of proteins, among which, CotE serves as a crucial morphogenetic protein within the outer coat. In this study, we observed that the homotrimeric CotE protein underwent further oligomerization induced by Ca\n              <jats:sup>2+<\/jats:sup>\n              and was subsequently dissociated by dipicolinic acid, a compound released from the spore core during germination. Through cryo-electron microscopy and tomography analyses of the Ca\n              <jats:sup>2+<\/jats:sup>\n              -induced CotE oligomer, combined with structural predictions and biochemical studies, we propose a three-dimensional meshwork organization facilitated by tryptophan-based interactions between CotE trimers. The resulting meshwork was organized in a defective diamond-like tetrahedral configuration. These insights enhance our understanding of how CotE contributes to endospore morphogenesis and germination through the rapid disassembly of these layers.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>Bacterial endospores are highly resilient structures that allow bacteria to survive extreme environmental conditions, making them a significant concern in food safety and healthcare. The protein CotE plays a critical role in forming the protective outer coat of these endospores. Our research uncovers the three-dimensional meshwork architecture of CotE and reveals how it contributes to the structural integrity and rapid disassembly of endospores during germination. By understanding CotE\u2019s unique 3D structure and its interaction with other molecules, we gain valuable insights into how bacterial endospores are formed and how they can be effectively targeted for sterilization. This work not only advances our fundamental knowledge of bacterial endospore biology but also has potential applications in developing new strategies to combat bacterial contamination and improve sterilization techniques in the food and healthcare industries.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.02472-24","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T14:00:36Z","timestamp":1741269636000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["3D meshwork architecture of the outer coat protein CotE: implications for bacterial endospore sporulation and germination"],"prefix":"10.1128","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2733-1953","authenticated-orcid":false,"given":"Dukwon","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Center for Food and Bioconvergence, Interdisciplinary Programs in Agricultural Genomics, CALS, Seoul National University","place":["Seoul, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeongjin","family":"Baek","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Center for Food and Bioconvergence, Interdisciplinary Programs in Agricultural Genomics, CALS, Seoul National University","place":["Seoul, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Migak","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Center for Food and Bioconvergence, Interdisciplinary Programs in Agricultural Genomics, CALS, Seoul National University","place":["Seoul, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doyeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Center for Food and Bioconvergence, Interdisciplinary Programs in Agricultural Genomics, CALS, Seoul National University","place":["Seoul, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyumi","family":"Byun","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University","place":["Seoul, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaekyung","family":"Hyun","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Sungkyunkwan University","place":["Suwon, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4813-748X","authenticated-orcid":true,"given":"Nam-Chul","family":"Ha","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Center for Food and Bioconvergence, Interdisciplinary Programs in Agricultural Genomics, CALS, Seoul National University","place":["Seoul, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12020248"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/elsc.202300013"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555819323.ch10"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-food-032519-051632"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00193-15"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/561975"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2921"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.63.1.1-20.1999"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.1999.0909"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2.8.1047"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-45770-6"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9084(92)90138-5"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13030"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/183124a0"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)45551-8"},{"key":"e_1_3_4_17_2","volume-title":"Brock biology of microorganisms","author":"Madigan MT","year":"2010","unstructured":"Madigan MT, Clark DP, Stahl D, Martinko JM. 2010. 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