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These drugs can be grouped in four distinct classes (azoles, candins, polyenes, and pyrimidine analogs) and are used in different clinical settings. Failures in therapy implicate the sequential or combined use of these different drug classes, which can result in some cases in the development of multidrug resistance (MDR). MDR is particularly challenging in the clinic since it drastically reduces possible treatment alternatives. In this study, we report the rapid development of MDR in\n                    <jats:named-content content-type=\"genus-species\">Candida lusitaniae<\/jats:named-content>\n                    in a patient, which became resistant to all known antifungal agents used until now in medicine. To understand how MDR developed in\n                    <jats:named-content content-type=\"genus-species\">C. lusitaniae<\/jats:named-content>\n                    , whole-genome sequencing followed by comparative genome analysis was undertaken in sequential MDR isolates. This helped to detect all specific mutations linked to drug resistance and explained the different MDR patterns exhibited by the clinical isolates.\n                  <\/jats:p>","DOI":"10.1128\/mbio.02512-19","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T07:54:55Z","timestamp":1578038095000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["Comparative Genomics for the Elucidation of Multidrug Resistance in Candida lusitaniae"],"prefix":"10.1128","volume":"10","author":[{"given":"Abhilash","family":"Kannan","sequence":"first","affiliation":[{"name":"Institute of Microbiology, Department of Laboratory, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra A.","family":"Asner","sequence":"additional","affiliation":[{"name":"Unit of Pediatric Infectious Diseases and Vaccinology, Department of 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