{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T23:58:13Z","timestamp":1790899093468,"version":"4.1.0"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["FCE_1_2014_1_104045"],"award-info":[{"award-number":["FCE_1_2014_1_104045"]}],"id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Comisi\u00f3n acad\u00e9mica de Posgrado, UdelaR"},{"name":"MERCOSUR Structural Convergence Found","award":["COF 03\/11"],"award-info":[{"award-number":["COF 03\/11"]}]},{"name":"ECOS-Sud France-Uruguay","award":["U20B02"],"award-info":[{"award-number":["U20B02"]}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-18-CE11-0017"],"award-info":[{"award-number":["ANR-18-CE11-0017"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["POS_NAC_2012_1_8824"],"award-info":[{"award-number":["POS_NAC_2012_1_8824"]}],"id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["POS_NAC_2015_1_109755"],"award-info":[{"award-number":["POS_NAC_2015_1_109755"]}],"id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["POS_FCE_2015_1_1005186"],"award-info":[{"award-number":["POS_FCE_2015_1_1005186"]}],"id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["POS_FCE_2020__1009183"],"award-info":[{"award-number":["POS_FCE_2020__1009183"]}],"id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100024018","name":"Programa de Desarrollo de las Ciencias B\u00e1sicas","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100024018","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,3,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Mycobacteria, including pathogens like\n                      <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                      , exhibit unique growth patterns and cell envelope structures that challenge our understanding of bacterial physiology. This study sheds light on FhaA, a conserved protein in\n                      <jats:italic>Mycobacteriales<\/jats:italic>\n                      , revealing its pivotal role in coordinating cell envelope biogenesis and asymmetric growth. The elucidation of the FhaA interactome in living mycobacterial cells reveals its participation in the protein network orchestrating cell envelope biogenesis and cell elongation\/division. By manipulating FhaA levels, we uncovered its influence on cell morphology, cell envelope organization, and the localization of peptidoglycan biosynthesis machinery. Notably,\n                      <jats:italic>fhaA<\/jats:italic>\n                      deletion disrupted the characteristic asymmetric growth of mycobacteria, highlighting its importance in maintaining this distinctive feature. Our findings position FhaA as a key regulator in a complex protein network, orchestrating the asymmetric distribution and activity of cell envelope biosynthetic machinery. This work not only advances our understanding of mycobacterial growth mechanisms but also identifies FhaA as a potential target for future studies on cell envelope biogenesis and bacterial growth regulation. These insights into the fundamental biology of mycobacteria may pave the way for novel approaches to combat mycobacterial infections addressing the ongoing challenge of diseases like tuberculosis in global health.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                      , the bacterium responsible for tuberculosis, remains a global health concern. Unlike most well-studied model bacilli, mycobacteria possess a distinctive and complex cell envelope, as well as an asymmetric polar growth mode. However, the proteins and mechanisms that drive cell asymmetric elongation in these bacteria are still not well understood. This study sheds light on the role of the protein FhaA in this process. Our findings demonstrate that FhaA localizes at the septum and asymmetrically to the poles, with a preference for the fast-growing pole. Furthermore, we showed that FhaA is essential for population heterogeneity and asymmetric polar elongation and plays a role in the precise subcellular localization of the cell wall biosynthesis machinery. Mycobacterial asymmetric elongation results in a physiologically heterogeneous bacterial population which is important for pathogenicity and response to antibiotics, stressing the relevance of identifying new factors involved in these still poorly characterized processes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.02526-24","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T09:01:32Z","timestamp":1737450092000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["FhaA plays a key role in mycobacterial polar elongation and asymmetric growth"],"prefix":"10.1128","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6475-9273","authenticated-orcid":false,"given":"Jessica","family":"Rossello","sequence":"first","affiliation":[{"name":"Analytical Biochemistry and Proteomics Unit, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable and Institut Pasteur de Montevideo","place":["Montevideo, Uruguay"]},{"name":"Advanced Bioimaging Unit, UdelaR and Institut Pasteur de Montevideo","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardina","family":"Rivera","sequence":"additional","affiliation":[{"name":"Analytical Biochemistry and Proteomics Unit, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable and Institut Pasteur de Montevideo","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximiliano","family":"Anzibar Fialho","sequence":"additional","affiliation":[{"name":"Advanced Bioimaging Unit, UdelaR and Institut Pasteur de Montevideo","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Augusto","sequence":"additional","affiliation":[{"name":"Precision Medicine Research Centre, Carlos Chagas Filho Institute of Biophysics and National Center for Structural Biology and Bioimaging, Federal University of Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Gil","sequence":"additional","affiliation":[{"name":"Analytical Biochemistry and Proteomics Unit, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable and Institut Pasteur de Montevideo","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Andrea","family":"Forrellad","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO), UEDD INTA-CONICET, CICVyA, Hurlingham","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Bigi","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda y Biolog\u00eda Molecular (IABIMO), UEDD INTA-CONICET, CICVyA, Hurlingham","place":["Buenos Aires, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azalia","family":"Rodr\u00edguez Ta\u00f1o","sequence":"additional","affiliation":[{"name":"Analytical Biochemistry and Proteomics Unit, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable and Institut Pasteur de Montevideo","place":["Montevideo, Uruguay"]},{"name":"Programa de Posgrado, Facultad de Qu\u00edmica, UdelaR","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefan\u00eda","family":"Urd\u00e1niz","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires","place":["Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Piuri","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires","place":["Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kildare","family":"Miranda","sequence":"additional","affiliation":[{"name":"Precision Medicine Research Centre, Carlos Chagas Filho Institute of Biophysics and National Center for Structural Biology and Bioimaging, Federal University of Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Marie","family":"Wehenkel","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, Bacterial Cell Cycle Mechanisms Unit","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4233-1903","authenticated-orcid":true,"given":"Pedro M.","family":"Alzari","sequence":"additional","affiliation":[{"name":"Institut Pasteur, CNRS UMR 3528, Universit\u00e9 Paris Cit\u00e9, Structural Microbiology Unit","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonel","family":"Malacrida","sequence":"additional","affiliation":[{"name":"Advanced Bioimaging Unit, UdelaR and Institut Pasteur de Montevideo","place":["Montevideo, Uruguay"]},{"name":"Departamento de Fisiopatolog\u00eda, Hospital de Cl\u00ednicas, Facultad de Medicina, UdelaR","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8575-9651","authenticated-orcid":true,"given":"Rosario","family":"Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Analytical Biochemistry and Proteomics Unit, Instituto de Investigaciones Biol\u00f3gicas Clemente Estable and Institut Pasteur de Montevideo","place":["Montevideo, Uruguay"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(22)00359-7"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3299"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1472-9792(02)00089-6"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1994.tb07194.x"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1402158111"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1216166"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00132"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2019.06.015"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008590"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2002525"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00963-06"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2007.08.006"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2011.07.011"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.09.004"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnw294"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2014.07.012"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.80395"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00850-19"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01005-12"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02207-14"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.772202"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1052"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","unstructured":"Hallgren J Tsirigos KD Pedersen MD Almagro Armenteros JJ Marcatili P Nielsen H Krogh A Winther O. 2022. DeepTMHMM predicts alpha and beta transmembrane proteins using deep neural networks. Bioinformatics. doi:10.1101\/2022.04.08.487609","DOI":"10.1101\/2022.04.08.487609"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1525165113"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.110154"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-47159-8"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-9792(13)70006-4"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20020107"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048211"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-019-01370-9"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000952"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.15698\/mic2019.02.667"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1080\/08927010903531491"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-08564-z"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.06.004"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01307-5"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00218-18"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.197"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00726-07"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044582"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-14088-z"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00649-17"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.0c01065"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00009-10"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/014076-0"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005010"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-327"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00721-0"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2018.09.013"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4074"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-023-01473-0"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15490-8"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-09926-3"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-022-00690-x"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.133"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts247"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1980.tb05599.x"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00291.x"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22489"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-012-1002-8"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2016.02.014"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2300942120"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1038"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.02526-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.02526-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T09:03:40Z","timestamp":1741770220000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mbio.02526-24"}},"subtitle":[],"editor":[{"given":"Edward W.","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,3,12]]},"references-count":63,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3,12]]}},"alternative-id":["10.1128\/mbio.02526-24"],"URL":"https:\/\/doi.org\/10.1128\/mbio.02526-24","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.08.13.607006","asserted-by":"object"}]},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,12]]},"assertion":[{"value":"2024-08-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02526-24"}}