{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:47:22Z","timestamp":1790509642036,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T00:00:00Z","timestamp":1574121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T00:00:00Z","timestamp":1577145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM099471-01A1"],"award-info":[{"award-number":["R01GM099471-01A1"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2019,12,24]]},"abstract":"<jats:p>\n                    Biofilm formation by the bacterial pathogen\n                    <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n                    is known to contribute to drug resistance in nosocomial infections and chronic lung infections of cystic fibrosis patients. In order to treat these infections more successfully, the mechanisms of bacterial biofilm development must be elucidated. While both bacterially secreted aminopeptidase and outer membrane vesicles have been shown to be abundant in\n                    <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n                    biofilm matrices, the contributions of each of these factors to the steps in biofilm generation have not been well studied. This work provides new insight into how these bacterial components mediate the formation of a robust, drug-resistant extracellular matrix and implicates outer membrane vesicles as active components of biofilm architecture, expanding our overall understanding of\n                    <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n                    biofilm biology.\n                  <\/jats:p>","DOI":"10.1128\/mbio.02548-19","type":"journal-article","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T08:41:38Z","timestamp":1574066498000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":82,"title":["Pseudomonas aeruginosa Leucine Aminopeptidase Influences Early Biofilm Composition and Structure via Vesicle-Associated Antibiofilm Activity"],"prefix":"10.1128","volume":"10","author":[{"given":"Caitlin N.","family":"Esoda","sequence":"first","affiliation":[{"name":"Duke University, Department of Molecular Genetics and Microbiology, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0519-3019","authenticated-orcid":false,"given":"Meta J.","family":"Kuehn","sequence":"additional","affiliation":[{"name":"Duke University, Department of Molecular Genetics and Microbiology, Durham, North Carolina, USA"},{"name":"Duke University, Department of Biochemistry, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12033"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00039"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.64.4.847-867.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00322.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens3030596"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00167"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnv117"},{"key":"e_1_3_2_9_2","first-page":"383","volume-title":"Current research, technology and education topics in applied microbiology and microbial biotechnology","author":"Hoge R","year":"2010","unstructured":"Hoge R, Pelzer A, Rosenau F, Wilhelm S, Mendez-Vilas A. 2010. 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