{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:26:48Z","timestamp":1787988408792,"version":"build-2784847793"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["NIH NIAID K25AI119229"],"award-info":[{"award-number":["NIH NIAID K25AI119229"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["NIH NIAID R01AI148489"],"award-info":[{"award-number":["NIH NIAID R01AI148489"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["NIH NIAID P01 AI27338"],"award-info":[{"award-number":["NIH NIAID P01 AI27338"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["NIH NIAID P01 AI27338"],"award-info":[{"award-number":["NIH NIAID P01 AI27338"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI123690"],"award-info":[{"award-number":["R21AI123690"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["NIH NIGMS R01GM087221"],"award-info":[{"award-number":["NIH NIGMS R01GM087221"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["NIH S10OD026936"],"award-info":[{"award-number":["NIH S10OD026936"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["NSF DBI-1920268"],"award-info":[{"award-number":["NSF DBI-1920268"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2021,12,21]]},"abstract":"<jats:p>Transmission of the malaria parasite to the mosquito vector is critical for the completion of the sexual stage of the parasite life cycle and is dependent on the release of male gametes from the gametocyte body inside the mosquito midgut. In the present study, we demonstrate that PfCDPK4 is critical for male gametogenesis and is involved in phosphorylation of proteins essential for male gamete emergence.<\/jats:p>","DOI":"10.1128\/mbio.02575-21","type":"journal-article","created":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T04:30:48Z","timestamp":1635827448000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Plasmodium falciparum Calcium-Dependent Protein Kinase 4 is Critical for Male Gametogenesis and Transmission to the Mosquito Vector"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2847-393X","authenticated-orcid":true,"given":"Sudhir","family":"Kumar","sequence":"first","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meseret T.","family":"Haile","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7029-7792","authenticated-orcid":true,"given":"Michael R.","family":"Hoopmann","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9626-9439","authenticated-orcid":true,"given":"Linh T.","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3386-5233","authenticated-orcid":true,"given":"Samantha A.","family":"Michaels","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6454-8970","authenticated-orcid":true,"given":"Seamus R.","family":"Morrone","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2007-2170","authenticated-orcid":true,"given":"Kayode K.","family":"Ojo","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, Center for Emerging and Reemerging Infectious Diseases (CERID), University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura M.","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6162-7577","authenticated-orcid":true,"given":"Ulrike","family":"Kusebauch","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5815-756X","authenticated-orcid":true,"given":"Ashley M.","family":"Vaughan","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, Washington, USA"},{"name":"Department of Pediatrics, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3216-9447","authenticated-orcid":true,"given":"Robert L.","family":"Moritz","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1540-1731","authenticated-orcid":true,"given":"Stefan H. I.","family":"Kappe","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, Washington, USA"},{"name":"Department of Global Health, University of Washington, Seattle, Washington, USA"},{"name":"Department of Pediatrics, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6756-4471","authenticated-orcid":true,"given":"Kristian E.","family":"Swearingen","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.10.020"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M085589"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.091208.073403"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/s0031182000048861"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0065-308x(08)60462-5"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/32667"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.20.12003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00449-0"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abe5396"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.05.017"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI61822"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit522"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13466"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500926"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1679"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00053-1"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00921-19"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.12-203877"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M900656200"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.09.004"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.26524"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1177\/0963689719884888"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1188191"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1715443115"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01656-17"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.561670"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05189.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.10.002"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0060139"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-68717-5"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.2000.00889.x"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04313.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008131"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.201900488"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.1038"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2011.00036"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn814"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.24.12020"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-13-315"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06733-w"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00336-09"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.135589"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.3791\/61426"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90782-6"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2377"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200439"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01097"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1049"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201400164"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.R113.027797"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.9b00205"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.031591"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty1058"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp335"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.02575-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mBio.02575-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T23:00:15Z","timestamp":1646694015000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mBio.02575-21"}},"subtitle":[],"editor":[{"given":"Louis M.","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,12,21]]},"references-count":55,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12,21]]}},"alternative-id":["10.1128\/mBio.02575-21"],"URL":"https:\/\/doi.org\/10.1128\/mbio.02575-21","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.06.06.447280","asserted-by":"object"}]},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,21]]},"assertion":[{"value":"2021-09-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02575-21"}}