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Successfully targeting the essential gene\n            <jats:italic>topA<\/jats:italic>\n            with a CRISPRi approach, using dCas12, in\n            <jats:italic>C. trachomatis<\/jats:italic>\n            indicates that this method will facilitate the characterization of the essential genome. These findings have an important impact on our understanding of the mechanisms by which well-balanced topoisomerase functions in adaptation of\n            <jats:italic>C. trachomatis<\/jats:italic>\n            to unfavorable growth conditions imposed by antibiotics.\n          <\/jats:p>","DOI":"10.1128\/mbio.02584-23","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T14:00:52Z","timestamp":1706104852000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Targeted repression of\n            <i>topA<\/i>\n            by CRISPRi reveals a critical function for balanced DNA topoisomerase I activity in the\n            <i>Chlamydia trachomatis<\/i>\n            developmental 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