{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:09:25Z","timestamp":1788368965530,"version":"build-2803163510"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82071663,81801486,82001567"],"award-info":[{"award-number":["82071663,81801486,82001567"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100019978","name":"Shanghai Pudong New Area Health Commission","doi-asserted-by":"crossref","award":["PWZzk2022-11"],"award-info":[{"award-number":["PWZzk2022-11"]}],"id":[{"id":"10.13039\/501100019978","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2024,12,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Although preterm birth (PTB) is one of the major causes of perinatal mortality and neonatal morbidity, little is known about its complex etiology. An abnormal cervicovaginal microbiome during pregnancy is associated with an increased risk of PTB. The cervicovaginal microbiota and its active metabolites, such as short-chain fatty acids (SCFAs), might be effectively used to predict and diagnose PTB. However, the roles of these proteins and the underlying mechanisms involved remain elusive. We conducted 16S rRNA gene sequencing and used a targeted metabolomics approach to study cervicovaginal swabs obtained from 51 singleton pregnancies and 52 twin pregnancies in the second trimester. Next, functional\n              <jats:italic>in vitro<\/jats:italic>\n              experiments were performed to investigate the roles and mechanisms of SCFAs in placental trophoblast cells (HTR8\/SVneo cells). Significant cervicovaginal microbiome dysbiosis, characterized by a substantial reduction in the abundance of lactobacilli and overgrowth of anaerobes, was revealed in the second trimester and was strongly associated with subsequent PTB (\n              <jats:italic>P<\/jats:italic>\n              = 0.036). Among the paired samples (\n              <jats:italic>n<\/jats:italic>\n              = 103), acetic acid was significantly greater in the preterm group than in the term group (\n              <jats:italic>P<\/jats:italic>\n              = 0.047). Data obtained from integrated gas chromatography\u2012mass spectrometry and 16S RNA studies revealed metabolites that were distinctly associated with particular microbial communities.\n              <jats:italic>Gardnerella vaginalis<\/jats:italic>\n              was the species most positively associated with acetic acid content. In addition, we identified a marker set consisting of the pregnancy type, acetic acid concentration, and community state type to accurately diagnose PTB. Acetate was associated with increased interleukin (IL)-8 and IL-6 levels and extravillous trophoblast cell migration and invasion through the activation of the extracellular signal-regulated kinase 1\/2 signaling pathway in HTR8\/SVneo cells. Cervicovaginal microbiota dysbiosis is an important etiological factor of PTB. The cervicovaginal microbiota and its active metabolites can be efficiently used to predict and diagnose PTB. Our findings enrich the microbiota\u2013placenta axis theory and contribute to the development of microecological products for pregnancy.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>Preterm birth (PTB) is a leading cause of infant mortality and long-term health issues, affecting millions of families worldwide. Despite its prevalence, the exact causes of PTB remain unclear. Our study reveals that certain bacteria and their metabolic byproducts in the cervicovaginal environment, specifically short-chain fatty acids (SCFAs), are linked to the risk of preterm birth. By analyzing samples from pregnant women, we found that an imbalance in the vaginal microbiota and increased levels of SCFAs are associated with changes in cells that can lead to early labor. This research provides new insights into how the microbiome influences pregnancy outcomes and highlights potential biomarkers for predicting preterm birth. Understanding these microbial influences could lead to innovative strategies for early diagnosis and prevention, ultimately improving maternal and infant health.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.02702-24","type":"journal-article","created":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T14:00:59Z","timestamp":1731333659000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Microbiome-producing SCFAs are associated with preterm birth via trophoblast function modulation"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1879-5203","authenticated-orcid":true,"given":"Lulu","family":"Meng","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Shanghai East Hospital, Tongji University School of Medicine","place":["Shanghai, China"]},{"name":"Clinical and Translational Research Center, Shanghai Key Laboratory of Maternal Fetal 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Translational Research Center, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwen","family":"Chang","sequence":"additional","affiliation":[{"name":"Clinical and Translational Research Center, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guihai","family":"Ai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Shanghai Tenth People\u2019s Hospital, School of Medicine, Tongji University","place":["Shanghai, 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