{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:46:39Z","timestamp":1791477999213,"version":"4.3.4"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T00:00:00Z","timestamp":1580774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T00:00:00Z","timestamp":1582588800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CRC\/TR124 FungiNet Project A3"],"award-info":[{"award-number":["CRC\/TR124 FungiNet Project A3"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2020,2,25]]},"abstract":"<jats:p>\n                    The use of animal models of infection is essential to advance our understanding of the complex host-pathogen interactions that take place during\n                    <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n                    lung infections. As in the case of humans, mice need to suffer an immune imbalance in order to become susceptible to invasive pulmonary aspergillosis (IPA), the most serious infection caused by\n                    <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n                    . There are several immunosuppressive regimens that are routinely used to investigate fungal growth and\/or immune responses in murine models of invasive pulmonary aspergillosis. However, the precise consequences of the use of each immunosuppressive model for the local immune populations and for fungal growth are not completely understood. Here, to pin down the scenarios involving commonly used IPA models, we employed light sheet fluorescence microscopy (LSFM) to analyze whole lungs at cellular resolution. Our results will be valuable to optimize and refine animal models to maximize their use in future research.\n                  <\/jats:p>","DOI":"10.1128\/mbio.02752-19","type":"journal-article","created":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T05:53:33Z","timestamp":1580709213000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Three-Dimensional Light Sheet Fluorescence Microscopy of Lungs To Dissect Local Host Immune-Aspergillus fumigatus Interactions"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8987-5115","authenticated-orcid":false,"given":"Jorge","family":"Amich","sequence":"first","affiliation":[{"name":"Department of Medicine II and IZKF Research Laboratory, W\u00fcrzburg University Hospital, W\u00fcrzburg, Germany"},{"name":"Research Center for Infectious Diseases, Julius-Maximilians-University W\u00fcrzburg, W\u00fcrzburg, 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