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The type III secretion system (T3SS) plays a key role in the pathogenesis of acute\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      infections. Expression of the T3SS is controlled by a master activator ExsA, whose transcription is driven by its operon promoter P\n                      <jats:italic toggle=\"yes\">\n                        <jats:sub>exsC<\/jats:sub>\n                      <\/jats:italic>\n                      as well as its own promoter P\n                      <jats:italic toggle=\"yes\">\n                        <jats:sub>exsA<\/jats:sub>\n                      <\/jats:italic>\n                      . Here, we found that plasmid replication initiation protein TrfA acts as a repressor for the T3SS gene expression in\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      . Production of the TrfA reduced the expression of the T3SS genes and the bacterial pathogenicity in a mouse acute pneumonia model. Further analysis revealed that the expression of\n                      <jats:italic toggle=\"yes\">trfA<\/jats:italic>\n                      resulted in upregulation of the novel gene PA5530, which mediates reduced cAMP production and decreased the expression of T3SS in PAK. In addition, we demonstrate that TrfA regulates the T3SS by directly binding to and modulating the P\n                      <jats:italic toggle=\"yes\">\n                        <jats:sub>exsC<\/jats:sub>\n                      <\/jats:italic>\n                      promoter. Of note, the clinical plasmid harboring the\n                      <jats:italic toggle=\"yes\">trfA<\/jats:italic>\n                      gene also leads to downregulation of T3SS in\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      . Overall, the results from this study reveal the effect of the plasmid replication initiation protein on the expression of its host chromosomal genes.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        As extrachromosomal elements, plasmids are well known for their role in conferring advantageous attributes to hosts, including antibiotic resistance, heavy metal resistance, the ability to degrade xenobiotics, and osmotolerance. Although several host chromosomally encoded proteins are required for the replication and maintenance of plasmids in their hosts, few proteins from plasmids have been reported to affect the host chromosomal gene expression. In this study, we identified TrfA, a plasmid replication initiation protein, as a novel repressor of the T3SS in\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        . We also elucidated the regulatory mechanism of T3SS repression mediated by the TrfA. The significance of this work is in the identification of the replication initiation protein of a naturally occurring plasmid from\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        functioning as a regulator of the expression of chromosomal genes in its host\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.02784-25","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T12:25:31Z","timestamp":1762345531000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Plasmid replication initiator protein TrfA represses the host type III secretion system in\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"16","author":[{"given":"Yu","family":"Zhang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwen","family":"Yin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University","place":["Tianjin, 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