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However, the role of the cervical bacterial microbiome in cervical cancer is not clear. To investigate that possibility, we collected cervical cytobrush samples from 144 Tanzanian women and performed deep sequencing of bacterial 16S rRNA genes. We found that HIV-positive patients had greater bacterial richness (\n            <jats:italic>P<\/jats:italic>\n            = 0.01) than HIV-negative patients. We also observed that women with high-grade squamous intraepithelial lesions (HSIL) had greater cervical bacterial diversity than women with cytologically normal cervices. Data from our precise sampling of cervical lesions leads us to propose that\n            <jats:italic>Mycoplasma<\/jats:italic>\n            contributes to a cervical microbiome status that promotes HPV-related cervical lesions. These results suggest a greater influence of the bacterial microbiota on the outcome of HPV infection than previously thought.\n          <\/jats:p>","DOI":"10.1128\/mbio.02785-18","type":"journal-article","created":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T14:28:28Z","timestamp":1550500108000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":69,"title":["Relationship between the Cervical Microbiome, HIV Status, and Precancerous Lesions"],"prefix":"10.1128","volume":"10","author":[{"given":"Cameron","family":"Klein","sequence":"first","affiliation":[{"name":"Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kandali","family":"Samwel","sequence":"additional","affiliation":[{"name":"Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crispin","family":"Kahesa","sequence":"additional","affiliation":[{"name":"Ocean Road Cancer Institute, Dar es Salaam, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julius","family":"Mwaiselage","sequence":"additional","affiliation":[{"name":"Ocean Road Cancer Institute, Dar es Salaam, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirosh","family":"Aluthge","sequence":"additional","affiliation":[{"name":"Animal Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samodha","family":"Fernando","sequence":"additional","affiliation":[{"name":"Animal Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John T.","family":"West","sequence":"additional","affiliation":[{"name":"Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Wood","sequence":"additional","affiliation":[{"name":"Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.","family":"Angeletti","sequence":"additional","affiliation":[{"name":"Nebraska Center for Virology, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.07.055"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.07.092"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/498048"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1159\/000442512"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm4040579"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2014.00048"},{"key":"e_1_3_2_8_2","first-page":"133","article-title":"Epidemiology of cervical squamous intraepithelial lesions in HIV infected women in Kenya: a cross-sectional study","volume":"19","author":"Memiah P","year":"2015","unstructured":"Memiah P, Makokha V, Mbuthia W, Kiiru GW, Agbor S, Odhiambo F, Ojoo S, Mbizo J, Muhula S, Mahasi G, Biadgilign S. 2015. 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