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During infection,\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            undergoes adaptive changes to evade host defenses and therapeutic interventions, increasing mortality and morbidity. Lipid A structural alteration is one such change that\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            isolates undergo during chronic lung infection in CF. Investigating genetic drivers of this lipid A structural variation is crucial in understanding\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            adaptation during infection. Here, we describe two lipid A dioxygenases with acyl-chain site specificity, each with different evolutionary origins. Further, we show that loss of function in these enzymes occurs in CF clinical isolates, suggesting a potential pathoadaptive phenotype. Studying these bacterial adaptations provides insight into selection pressures of the CF airway on\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            phenotypes that persist during chronic infection. Understanding these adaptive changes may ultimately provide clinicians better control over bacterial populations during chronic infection.\n          <\/jats:p>","DOI":"10.1128\/mbio.02823-23","type":"journal-article","created":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T13:38:28Z","timestamp":1703252308000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Divergent\n            <i>Pseudomonas aeruginosa<\/i>\n            LpxO enzymes perform site-specific lipid A 2-hydroxylation"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2798-1382","authenticated-orcid":false,"given":"Casey E.","family":"Hofstaedter","sequence":"first","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland, Baltimore, Baltimore, Maryland, USA"},{"name":"Medical Scientist Training Program, University of Maryland, Baltimore, Baltimore, Maryland, 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M.","family":"Harro","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland, Baltimore, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Goodlett","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Microbiology, University of Victoria, Victoria, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7337-7154","authenticated-orcid":true,"given":"David A.","family":"Rasko","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Maryland Baltimore, Baltimore, Maryland, USA"},{"name":"Institute for Genome Sciences, University of Maryland, Baltimore, Baltimore, Maryland, USA"},{"name":"Center for Pathogen Research, University of Maryland, Baltimore, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5016-8694","authenticated-orcid":true,"given":"Robert K.","family":"Ernst","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland, Baltimore, Baltimore, Maryland, USA"},{"name":"Department of Microbiology and Immunology, University of Maryland Baltimore, Baltimore, Maryland, USA"},{"name":"Center for Pathogen Research, University of Maryland, Baltimore, Baltimore, Maryland, 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