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This bacterial transfer process depends on the host actin cytoskeleton as the bacterium forms motile actin-rich membranous protrusions that propel the bacteria into neighboring cells, thus forming corresponding membrane invaginations. Here, we examine these membrane invaginations and demonstrate that caveolin-1\u2013based endocytosis is crucial for efficient bacterial cell-to-cell spreading. We show that only a subset of caveolin-associated proteins (cavin-2 and EHD2) are involved in this process. Despite the absence of clathrin at the invaginations, the classical clathrin-associated protein epsin-1 is also required for efficient bacterial spreading. Using isolated\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            protrusions added onto naive host cells, we demonstrate that actin-based propulsion is dispensable for caveolin-1 endocytosis as the presence of the protrusion\/invagination interaction alone triggers caveolin-1 recruitment in the recipient cells. Finally, we provide a model of how this caveolin-1\u2013based internalization event can exceed the theoretical size limit for this endocytic pathway.\n          <\/jats:p>","DOI":"10.1128\/mbio.02857-19","type":"journal-article","created":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T09:38:05Z","timestamp":1579513085000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Listeria monocytogenes Exploits Host Caveolin for Cell-to-Cell Spreading"],"prefix":"10.1128","volume":"11","author":[{"given":"Aaron S.","family":"Dhanda","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Centre for Cell Biology, Development, and Disease, Simon Fraser University, Burnaby, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connie","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Centre for Cell Biology, Development, and Disease, Simon Fraser University, Burnaby, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina T.","family":"Lulic","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Centre for Cell Biology, Development, and Disease, Simon Fraser University, Burnaby, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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