{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T19:18:09Z","timestamp":1789067889696,"version":"build-2803163510"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["91951202"],"award-info":[{"award-number":["91951202"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"MOST | National Key Research and Development Program of China","award":["2022YFC3401301"],"award-info":[{"award-number":["2022YFC3401301"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2024,3,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Methanethiol (MT) is a sulfur-containing compound produced during dimethylsulfoniopropionate (DMSP) degradation by marine bacteria. The C\u2013S bond of MT can be cleaved by methanethiol oxidases (MTOs) to release a sulfur atom. However, the cleaving process remains unclear, and the species of sulfur product is uncertain. It has long been assumed that MTOs produce hydrogen sulfide (H\n              <jats:sub>2<\/jats:sub>\n              S) from MT. Herein, we studied the MTOs in the Rhodobacteraceae family\u2014whose members are important DMSP degraders ubiquitous in marine environments. We identified 57 MTOs from 1,904 Rhodobacteraceae genomes. These MTOs were grouped into two major clusters. Cluster 1 members share three conserved cysteine residues, while cluster 2 members contain one conserved cysteine residue. We examined the products of three representative MTOs both\n              <jats:italic>in vitro<\/jats:italic>\n              and\n              <jats:italic>in vivo<\/jats:italic>\n              . All of them produced sulfane sulfur other than H\n              <jats:sub>2<\/jats:sub>\n              S from MT. Their conserved cysteines are substrate-binding sites in which the MTO\u2013S\u2013S\u2013CH\n              <jats:sub>3<\/jats:sub>\n              complex is formed. This finding clarified the sulfur product of MTOs and enlightened the MTO-catalyzing process. Moreover, this study connected DMSP degradation with sulfane sulfur metabolism, filling a critical gap in the DMSP degradation pathway and representing new knowledge in the marine sulfur cycle field.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              This study overthrows a long-time assumption that methanethiol oxidases (MTOs) cleave the C\u2013S bond of methanethiol to produce both H\n              <jats:sub>2<\/jats:sub>\n              S and H\n              <jats:sub>2<\/jats:sub>\n              O\n              <jats:sub>2<\/jats:sub>\n              \u2014the former is a strong reductant and the latter is a strong oxidant. From a chemistry viewpoint, this reaction is difficult to happen. Investigations on three representative MTOs indicated that sulfane sulfur (S\n              <jats:sup>0<\/jats:sup>\n              ) was the direct product, and no H\n              <jats:sub>2<\/jats:sub>\n              O\n              <jats:sub>2<\/jats:sub>\n              was produced. Finally, the products of MTOs were corrected to be S\n              <jats:sup>0<\/jats:sup>\n              and H\n              <jats:sub>2<\/jats:sub>\n              O. This finding connected dimethylsulfoniopropionate (DMSP) degradation with sulfane sulfur metabolism, filling a critical gap in the DMSP degradation pathway and representing new knowledge in the marine sulfur cycle field.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.02907-23","type":"journal-article","created":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T14:01:17Z","timestamp":1707400877000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Rhodobacteraceae methanethiol oxidases catalyze methanethiol degradation to produce sulfane sulfur other than hydrogen sulfide"],"prefix":"10.1128","volume":"15","author":[{"given":"Qun","family":"Cao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanyu","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingda","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongzheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Qingdao Institute of Animal Husbandry and Veterinary Medicine, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9950-1910","authenticated-orcid":true,"given":"Yongzhen","family":"Xia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luying","family":"Xun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China"},{"name":"School of Molecular Biosciences, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0483-5318","authenticated-orcid":true,"given":"Huaiwei","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf7796"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0119-5"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-018-9524-y"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1385-1101(00)00023-X"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.12.5804-5815.2002"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.64045"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-021-01153-3"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0675-0"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4203(96)00006-0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1130657"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10078"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2017.148"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.16045"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2003.12.017"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01040"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-002-8450-6"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.12.4741-4747.1997"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1029\/2000JD900571"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1029\/2000JD900630"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.10.4549-4558.1999"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-138-1-217"},{"key":"e_1_3_3_23_2","first-page":"2989","article-title":"Methyl mercaptan oxidase, a key enzyme in the metabolism of methylated sulfur-compounds by hyphomicrobium EG","volume":"133","author":"Suylen GMH","year":"1987","unstructured":"Suylen GMH, Large PJ, Vandijken JP, Kuenen JG. 1987. 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