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An alternative to antibiotics is the therapeutic use of bacteriophages (phages). There are recent cases of phage therapy being used to treat\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    infections in people under compassionate-use conditions. However, little is known about the ability of phages to kill bacteria, such as\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    , which reside in an intracellular environment. Here, we used\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    strains and phages from recent phage therapy cases to determine if phages can enter mammalian cells and if they can infect and kill intracellular\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    . Using fluorescence microscopy, we demonstrate phage uptake by macrophages and lung epithelial cells, and we further demonstrate phage infection of intracellular\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    with fluorescent reporter phages. Transmission electron microscopy was additionally used to image phage infection of intracellular\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    . Together, these findings provide the first visualizations of phage-\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    interactions in an intracellular environment. Finally, we show that phage treatment can significantly reduce the intracellular burden of\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    in a manner that depends on both the specific phage and mammalian cell type involved. These results demonstrate the potential to use phage therapy to treat intracellular bacteria, specifically\n                    <jats:italic>M. abscessus<\/jats:italic>\n                    , while also highlighting the importance of prescreening phage therapy candidates for activity in an intracellular environment.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      As we rapidly approach a post-antibiotic era, bacteriophage (phage) therapy may offer a solution for treating drug-resistant bacteria.\n                      <jats:italic>Mycobacterium abscessus<\/jats:italic>\n                      is an emerging, multidrug-resistant pathogen that causes disease in people with cystic fibrosis, chronic obstructive pulmonary disease, and other underlying lung diseases.\n                      <jats:italic>M. abscessus<\/jats:italic>\n                      can survive inside host cells, a niche that can limit access to antibiotics. As current treatment options for\n                      <jats:italic>M. abscessus<\/jats:italic>\n                      infections often fail, there is an urgent need for alternative therapies. Phage therapy is being used to treat\n                      <jats:italic>M. abscessus<\/jats:italic>\n                      infections as an option of last resort. However, little is known about the ability of phages to kill bacteria in the host environment and specifically in an intracellular environment. Here, we demonstrate the ability of phages to enter mammalian cells and to infect and kill intracellular\n                      <jats:italic>M. abscessus<\/jats:italic>\n                      . These findings support the use of phages to treat intracellular bacterial pathogens.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.02924-23","type":"journal-article","created":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T09:00:56Z","timestamp":1701939656000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Bacteriophage infection and killing of intracellular\n                    <i>Mycobacterium abscessus<\/i>"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9100-4431","authenticated-orcid":false,"given":"Alan A.","family":"Schmalstig","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, UNC School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 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