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Here, we characterized an enterocolitis model of foodborne<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>infection. This work highlights a multiorgan trafficking circuit and reveals a fitness advantage for bacteria that successfully complete this cycle. Because virulence factors play critical roles in systemic dissemination and multiple bottlenecks occur as the bacterial population colonizes different tissue sites, this multiorgan trafficking circuit likely provides purifying selection of virulence genes. This study also serves as a foundation for future work using the<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>-induced enterocolitis model to investigate the biology of<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>in the intestinal environment.<\/jats:p>","DOI":"10.1128\/mbio.02948-19","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T12:54:47Z","timestamp":1576500887000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["A Multiorgan Trafficking Circuit Provides Purifying Selection of Listeria monocytogenes Virulence Genes"],"prefix":"10.1128","volume":"10","author":[{"given":"Alexander","family":"Louie","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Becattini","sequence":"additional","affiliation":[{"name":"Immunology Program, Sloan Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew K.","family":"Waldor","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Howard Hughes Medical Institute, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Portnoy","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA"},{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200004273421702"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30521-7"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70870-9"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.4.1459"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90188-i"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406035101"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"2266","DOI":"10.4049\/jimmunol.138.7.2266","article-title":"Intracellular growth of Listeria monocytogenes as a prerequisite for in vivo induction of T cell-mediated immunity","volume":"138","author":"Berche P","year":"1987","unstructured":"Berche P, Gaillard JL, Sansonetti P. 1987. 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