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Despite its usefulness, a CRISPR system had yet to be developed for use in\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            (Gc), a bacterium that is the main etiological agent of gonorrhea infection. Here, we developed a programmable and IPTG-inducible Type I-C CRISPR interference (CRISPRi) system derived from the commensal species\n            <jats:italic>Neisseria lactamica<\/jats:italic>\n            as a gene repression system in Gc. As opposed to generating genetic knockouts, the Type I-C CRISPRi system allows us to block transcription of specific genes without generating deletions in the DNA. We explored the properties of this system and found that a minimal spacer array is sufficient for gene repression while also facilitating efficient spacer reprogramming. Importantly, we also show that we can use CRISPRi to knockdown genes that are essential to Gc that cannot normally be knocked out under laboratory settings. Gc encodes ~800 essential genes, many of which have no predicted function. We predict that this Type I-C CRISPRi system can be used to help categorize gene functions and perhaps contribute to the development of novel therapeutics for gonorrhea.\n          <\/jats:p>","DOI":"10.1128\/mbio.03025-23","type":"journal-article","created":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T14:00:41Z","timestamp":1703167241000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Development and implementation of a Type I-C CRISPR-based programmable repression system for\n            <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1128","volume":"15","author":[{"given":"Wendy E.","family":"Geslewitz","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Northwestern University, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaris","family":"Cardenas","sequence":"additional","affiliation":[{"name":"Department of 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