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The assembly of the nitrogenase [7Fe-9S-C-Mo-\n            <jats:italic>R<\/jats:italic>\n            -homocitrate]-cofactor (FeMo-co) in a eukaryotic cell is a pivotal milestone that will pave the way to engineer cereals with nitrogen fixing capabilities and therefore independent of nitrogen fertilizers. In this study, we identified NifEN protein complexes that were functional in the model eukaryotic organism\n            <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n            . NifEN is an essential component of the FeMo-co biosynthesis pathway. Furthermore, the FeMo-co biosynthetic pathway was recapitulated\n            <jats:italic>in vitro<\/jats:italic>\n            using only proteins expressed in\n            <jats:italic>S. cerevisiae<\/jats:italic>\n            . FeMo-co biosynthesis was achieved by combining nitrogenase FeMo-co assembly components from different species, a promising strategy to engineer nitrogen fixation in eukaryotic organisms.\n          <\/jats:p>","DOI":"10.1128\/mbio.03088-23","type":"journal-article","created":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T14:00:15Z","timestamp":1703167215000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Nitrogenase cofactor biosynthesis using proteins produced in mitochondria of\n            <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1128","volume":"15","author":[{"given":"Katarzyna","family":"Dobrzy\u0144ska","sequence":"first","affiliation":[{"name":"Centro de Biotecnolog\u00eda y Gen\u00f3mica de Plantas, Universidad Polit\u00e9cnica de Madrid (UPM), Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA\/CSIC), Pozuelo de Alarc\u00f3n, 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