{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T03:53:26Z","timestamp":1782532406865,"version":"3.54.5"},"reference-count":73,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | National Institutes of Health","award":["GM068087"],"award-info":[{"award-number":["GM068087"]}]},{"name":"HHS | National Institutes of Health","award":["GM086565"],"award-info":[{"award-number":["GM086565"]}]},{"name":"USDA | National Institute of Food and Agriculture","award":["CA-R-PPA-6980-H"],"award-info":[{"award-number":["CA-R-PPA-6980-H"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2024,12,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Heterotrimeric G protein signaling pathways control growth and development in eukaryotes. In the multicellular fungus\n              <jats:italic>Neurospora crassa<\/jats:italic>\n              , the guanine nucleotide exchange factor RIC8 regulates heterotrimeric G\u03b1 subunits. In this study, we used RNAseq and liquid chromatography-mass spectrometry (LC-MS) to profile the transcriptomes and metabolomes of\n              <jats:italic>N. crassa<\/jats:italic>\n              wild type, the G\u03b1 subunit mutants \u0394\n              <jats:italic>gna-1<\/jats:italic>\n              and \u0394\n              <jats:italic>gna-3<\/jats:italic>\n              , and \u0394\n              <jats:italic>ric8<\/jats:italic>\n              strains. These strains exhibit defects in growth and asexual development (conidiation), with wild-type and \u0394\n              <jats:italic>gna-1<\/jats:italic>\n              mutants producing hyphae in submerged cultures, while \u0394\n              <jats:italic>gna-3<\/jats:italic>\n              and \u0394\n              <jats:italic>ric8<\/jats:italic>\n              mutants develop conidiophores, particularly in the \u0394\n              <jats:italic>ric8<\/jats:italic>\n              mutant. RNAseq analysis showed that the \u0394\n              <jats:italic>gna-1<\/jats:italic>\n              mutant possesses 159 mis-regulated genes, while \u0394\n              <jats:italic>gna-3<\/jats:italic>\n              and \u0394\n              <jats:italic>ric8<\/jats:italic>\n              strains have more than 1,000 each. Many of the mis-regulated genes are involved in energy homeostasis, conidiation, or metabolism. LC-MS revealed changes in levels of primary metabolites in the mutants, with several arginine metabolic intermediates impacted in \u0394\n              <jats:italic>ric8<\/jats:italic>\n              strains. The differences in metabolite levels could not be fully explained by the expression or activity of pathway enzymes. However, transcript levels for two predicted vacuolar arginine transporters were affected in \u0394\n              <jats:italic>ric8<\/jats:italic>\n              mutants. Analysis of arginine and ornithine levels in transporter mutants yielded support for altered compartmentation of arginine and ornithine between the cytosol and vacuole in \u0394\n              <jats:italic>ric8<\/jats:italic>\n              strains. Furthermore, we validated previous reports that arginine and ornithine levels are low in wild-type conidia. Our results suggest that RIC8 regulates asexual sporulation in\n              <jats:italic>N. crassa<\/jats:italic>\n              at least in part through altered expression of vacuolar transporter genes and the resultant mis-compartmentation of arginine and ornithine.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Resistance to inhibitors of cholinesterase-8 (RIC8) is an important regulator of heterotrimeric G\u03b1 proteins in eukaryotes. In the filamentous fungus\n              <jats:italic>Neurospora crassa<\/jats:italic>\n              , mutants lacking ric8 undergo inappropriate asexual development (macroconidiation) during submerged growth. Our work identifies a role for RIC8 in regulating expression of transporter genes that retain arginine and ornithine in the vacuole (equivalent of the animal lysosome) and relates this function to the developmental defect. Arginine is a critical cellular metabolite, both as an amino acid for protein synthesis and as a precursor for an array of compounds, including proline, ornithine, citrulline, polyamines, creatine phosphate, and nitric oxide. These results have broad relevance to human physiology and disease, as arginine modulates immune, vascular, hormonal, and other functions in humans.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.03133-24","type":"journal-article","created":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T14:00:39Z","timestamp":1731938439000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["RNAseq and targeted metabolomics implicate RIC8 in regulation of energy homeostasis, amino acid compartmentation, and asexual development in\n            <i>Neurospora crassa<\/i>"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8888-7306","authenticated-orcid":false,"given":"Monique","family":"Quinn","sequence":"first","affiliation":[{"name":"Department of Microbiology and Plant Pathology, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander J.","family":"Carrillo","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Plant Pathology, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lida","family":"Halilovic","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Plant Pathology, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1112-6034","authenticated-orcid":true,"given":"Katherine A.","family":"Borkovich","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Plant Pathology, University of California","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.026"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.08.002"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.61.080706.093432"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20160819"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211862200"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.10.008"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/147.1.137"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.7.8.1283"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.1.3.378-390.2002"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.166.3.1229"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04096.x"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.111.136358"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.2.365-378.2005"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00124-06"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00109-06"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00151-12"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82405-X"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.20.7693-7705.2000"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0223334"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1534\/g3.115.020974"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.111.129270"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048026"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02451771"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00417852"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-5975(83)90049-X"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1139\/m84-192"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.8.6.2411-2418.1988"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/146.2.499"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.1.4.634-642.2002"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00231-10"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.1998.1101"},{"key":"e_1_3_5_33_2","volume-title":"Enzyme nomenclature 1992","author":"NCotIUoBaM B","year":"1992","unstructured":"NCotIUoBaM B. 1992. 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