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The former two toxins are known to be involved in the emergence of some clinical symptoms and\/or contribute to the establishment of bacterial infection. In contrast, the role of DNT in pertussis remains unclear. Our study shows that DNT affects neural cells through specific binding to the T-type voltage-gated Ca<jats:sup>2+<\/jats:sup>channel that is highly expressed in the central nervous system and leads to neurological disorders in mice after intracerebral injection. 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