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Like other apicomplexans, it possesses specialized secretory organelles in the zoites, in which micronemes discharge molecules to facilitate the movement and invasion of zoites. Although past and recent studies have identified several proteins in cryptosporidial micronemes, our understanding of the composition, secretory pathways, and domain-ligand interactions of micronemal proteins remains limited. This study identifies a new micronemal protein, namely, CpTSP4, that is discharged during excystation, gliding, and invasion of\n            <jats:italic>C. parvum<\/jats:italic>\n            sporozoites. The CpTSP4 secretion depends on the intracellular trafficking on the two\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            -unique microtubes that could be blocked by kinesin-5\/Eg5 inhibitors. Additionally, a novel heparin-binding motif is identified and biochemically validated, which contributes to the nanomolar binding affinity of CpTSP4 to host cells. These findings indicate that kinesin-dependent microtubular trafficking is critical to CpTSP4 secretion, and heparin\/heparan sulfate is one of the ligands for this micronemal protein.\n          <\/jats:p>","DOI":"10.1128\/mbio.03158-23","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T14:00:37Z","timestamp":1706104837000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Requirement of microtubules for secretion of a micronemal protein CpTSP4 in the invasive stage of the apicomplexan\n            <i>Cryptosporidium parvum<\/i>"],"prefix":"10.1128","volume":"15","author":[{"given":"Dongqiang","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin 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