{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:45:47Z","timestamp":1788572747151,"version":"build-2803163510"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["5R01AI160387"],"award-info":[{"award-number":["5R01AI160387"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT-2018-01892"],"award-info":[{"award-number":["PICT-2018-01892"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2026,1,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Trichomonas vaginalis<\/jats:italic>\n                      is a common sexually transmitted parasite that colonizes the human urogenital tract, causing infections that range from asymptomatic to highly inflammatory. As an extracellular pathogen, adherence to host epithelial cells is an important step to colonize the human host. Hence, understanding how the process of attachment to host cells is regulated remains an important goal in parasitology research and human health.\n                      <jats:italic toggle=\"yes\">T. vaginalis<\/jats:italic>\n                      -host interaction is regulated by changes in gene expression, but it is still largely unknown how these changes in transcriptional profiles are controlled, as very few transcriptional regulatory elements have been described. Our recent work highlighted the importance of epigenetics in the regulation of transcription, and a specific role for N6-methyladenine (6mA) in modulating three-dimensional chromatin structure has been suggested. Building on these findings, we analyzed here the role of 6mA and chromatin accessibility during the process of host-parasite interaction by integrating MeDIP-seq and assay for transposase-accessible chromatin sequencing data with RNA-seq in free vs host cell-attached parasites. Consistent with our previous results, we identified transcriptionally active and repressive regions flanked by 6mA modifications, observed both in the presence and absence of host cells. Importantly, we detected differentially accessible chromatin regions that influence the gene expression of key pathogenesis-related genes during\n                      <jats:italic toggle=\"yes\">T. vaginalis<\/jats:italic>\n                      host cell interaction. These findings highlight the importance of chromatin architecture in regulating gene expression during parasitic infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Trichomonas vaginalis<\/jats:italic>\n                      , the most common non-viral sexually transmitted parasite, relies on adherence to host epithelial cells to establish infection. Our previous work highlighted the importance of N6-methyladenine (6mA) DNA methylation in the regulation of transcription and three-dimensional chromatin structure. Now, our study integrates RNA-seq, MeDIP-seq, and assay for transposase-accessible chromatin sequencing data to reveal how 6mA and chromatin accessibility modulate gene expression during\n                      <jats:italic toggle=\"yes\">T. vaginalis<\/jats:italic>\n                      interaction with human host cells. We identified over 3,600 differentially expressed genes upon parasite contact with prostate cells, including pathogenesis-related genes. Moreover, we identified transcriptionally active and repressive regions flanked by 6mA that remain largely stable during the process of host interaction. We mapped genome-wide chromatin accessibility and uncovered differentially accessible regions upon host cell contact associated with a subset of genes involved in adhesion. These results suggest that local chromatin accessibility has a major role in modulating gene expression of key virulence genes during host interaction.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.03175-25","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T14:00:26Z","timestamp":1764770426000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Influence of DNA methylation and chromatin accessibility on regulation of gene expression during\n                    <i>Trichomonas vaginalis-<\/i>\n                    host cell interaction"],"prefix":"10.1128","volume":"17","author":[{"given":"Daniela","family":"Mu\u00f1oz","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04qzr9r50","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Par\u00e1sitos Anaerobios, Instituto Tecnol\u00f3gico Chascom\u00fas (INTECH), CONICET-UNSAM","place":["Chascom\u00fas, Argentina"]},{"name":"Escuela de Bio y Nanotecnolog\u00edas (UNSAM)","place":["Chascom\u00fas, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayel\u00e9n","family":"Lizarraga","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04qzr9r50","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Par\u00e1sitos Anaerobios, Instituto Tecnol\u00f3gico Chascom\u00fas (INTECH), CONICET-UNSAM","place":["Chascom\u00fas, Argentina"]},{"name":"Escuela de Bio y Nanotecnolog\u00edas (UNSAM)","place":["Chascom\u00fas, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4472-2702","authenticated-orcid":true,"given":"Patricia J.","family":"Johnson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/046rm7j60","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Immunology & Molecular Genetics, University of California","place":["Los Angeles, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo H.","family":"Strobl-Mazzulla","sequence":"additional","affiliation":[{"name":"Escuela de Bio y Nanotecnolog\u00edas (UNSAM)","place":["Chascom\u00fas, Argentina"]},{"id":[{"id":"https:\/\/ror.org\/04qzr9r50","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Biolog\u00eda del Desarrollo, Instituto Tecnol\u00f3gico Chascom\u00fas (INTECH), CONICET-UNSAM","place":["Chascom\u00fas, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3864-0703","authenticated-orcid":true,"given":"Natalia","family":"de Miguel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04qzr9r50","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Par\u00e1sitos Anaerobios, Instituto Tecnol\u00f3gico Chascom\u00fas (INTECH), CONICET-UNSAM","place":["Chascom\u00fas, Argentina"]},{"name":"Escuela de Bio y Nanotecnolog\u00edas (UNSAM)","place":["Chascom\u00fas, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","unstructured":"WHO. 2021. 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