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How to improve the efficacy of existing antibiotics is of importance for combating antibiotic-resistant\/tolerant pathogens. Here, we report that 10-s rapid freezing with liquid nitrogen dramatically enhanced the bactericidal action of aminoglycoside antibiotics by 2 to 6 orders of magnitude against many bacterial pathogens\n                    <jats:italic>in vitro<\/jats:italic>\n                    and also in a mouse skin wound model. In particular, such combined treatment was able to effectively kill persister cells of\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa<\/jats:named-content>\n                    , which are\n                    <jats:italic>per se<\/jats:italic>\n                    tolerant of conventional treatment with bactericidal antibiotics for several hours. We also demonstrated that freezing-induced aminoglycoside potentiation was apparently linked to freezing-induced cell membrane damage that may have activated the mechanosensitive ion channel MscL, which, in turn, was able to effectively uptake aminoglycoside antibiotics in a proton motive force-independent manner. Our report sheds light on the development of a new strategy against bacterial pathogens by combining existing antibiotics with a conventional physical treatment or with MscL agonists.\n                  <\/jats:p>","DOI":"10.1128\/mbio.03239-19","type":"journal-article","created":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T06:45:37Z","timestamp":1581317137000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Rapid Freezing Enables Aminoglycosides To Eradicate Bacterial Persisters via Enhancing Mechanosensitive Channel MscL-Mediated Antibiotic Uptake"],"prefix":"10.1128","volume":"11","author":[{"given":"Yanna","family":"Zhao","sequence":"first","affiliation":[{"name":"Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal 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