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Despite its huge global impact upon human health, our understanding of the pathobiology of this deadly fungus remains extremely limited, largely because it is not yet possible to cultivate\n              <jats:italic>Pneumocystis in vitro,<\/jats:italic>\n              independently of the host. However, a recent paper by Munyonho et al. offers a major step forward (F. T. Munyonho, R. D. Clark, D. Lin, M. S. Khatun, et al., 2023, mBio 15:e01464-23,\n              <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1128\/mbio.01464-23\" xlink:type=\"simple\">https:\/\/doi.org\/10.1128\/mbio.01464-23<\/jats:ext-link>\n              ). They show that it is possible to maintain both the trophozoite and cyst forms of the mouse pathogen,\n              <jats:italic>Pneumocystis murina,<\/jats:italic>\n              in precision-cut lung slices for several weeks. Furthermore, they demonstrate that this offers the exciting opportunity to examine potential virulence factors such as possible biofilm formation as well as antifungal drug responses in the lung.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.03277-23","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T14:07:54Z","timestamp":1707746874000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Slicing through the challenge of maintaining\n            <i>Pneumocystis<\/i>\n            in the laboratory"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0055-2211","authenticated-orcid":true,"given":"Olga A.","family":"Nev","sequence":"first","affiliation":[{"name":"Biosciences and Living Systems Institute, University of Exeter, Exeter, United Kingdom"},{"name":"Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucian","family":"Duvenage","sequence":"additional","affiliation":[{"name":"Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom"},{"name":"CMM AFRICA Medical Mycology Research Unit, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa"},{"name":"Department of Pathology, Division of Immunology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1406-4251","authenticated-orcid":true,"given":"Alistair J. 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