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While\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      is known to exhibit a robust competitive response toward other bacterial species, this bacterium is frequently identified in polymicrobial infections where multiple species survive. For example, in prosthetic joint infections,\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      can be identified along with other pathogenic bacteria including\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      ,\n                      <jats:italic>Enterococcus faecalis<\/jats:italic>\n                      , and\n                      <jats:italic>Corynebacterium striatum<\/jats:italic>\n                      . Here, we have explored the survival and behavior of such microbes and find that\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      readily survives culturing with\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      while other tested species do not. In each of the tested conditions,\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      growth remained unchanged by the presence of\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      , indicating a unique mutualistic interaction between the two species. We find that\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      proximity leads\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      to attenuate competitive behaviors as exemplified by reduced production of\n                      <jats:italic>Pseudomonas<\/jats:italic>\n                      quinolone signal and pyocyanin. Reduced alkyl quinolones are important to\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      as these will grow in supernatant from a quinolone mutant but not\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      wild-type in planktonic culture. The reduced pyocyanin production of\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      is attributable to production of ornithine by\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      , which we recapitulate by adding exogenous ornithine to\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      monocultures. Similarly, co-culture with an ornithine-deficient strain of\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      leads\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      to yield near monoculture amounts of pyocyanin. Here, we directly demonstrate how notorious pathogens such as\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      might persist in polymicrobial infections under the influence of metabolites produced by other bacterial species.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      While we now appreciate that many infections are polymicrobial, we understand little of the specific actions between a given set of microbes to enable combinatorial survival and pathogenesis. The bacteria\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      and\n                      <jats:italic>Enterococcus faecalis<\/jats:italic>\n                      are both prevalent pathogens in wound, urinary tract, and bacteremic infections. While\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      often kills other species in standard laboratory culture conditions, we present here that\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      can be reliably co-cultured with\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      . We specifically detail that ornithine produced by\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      reduces the\n                      <jats:italic>Pseudomonas<\/jats:italic>\n                      quinolone signal response of\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      . This reduction of the\n                      <jats:italic>Pseudomonas<\/jats:italic>\n                      quinolone signal response aids\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      growth.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.03320-24","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T09:00:21Z","timestamp":1735290021000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["<i>Pseudomonas aeruginosa<\/i>\n                    alkyl quinolone response is dampened by\n                    <i>Enterococcus faecalis<\/i>"],"prefix":"10.1128","volume":"16","author":[{"given":"Maggie M.","family":"Fink","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Notre Dame","place":["Notre Dame, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail A.","family":"Weaver","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame","place":["Notre Dame, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dharmeshkumar","family":"Parmar","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign","place":["Urbana, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8286-3808","authenticated-orcid":true,"given":"Jon E.","family":"Paczkowski","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University at Albany, School of Public Health","place":["Albany, USA"]},{"name":"Division of Genetics, Wadsworth Center, New York State Department of Health","place":["Albany, 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